Optical trapping in plant cells

Tijs Ketelaar*, Norbert de Ruijter, Stefan Niehren

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Optical tweezers allow for noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe experimental approaches we have taken to trap intracellular structures in plant cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationMethods and protocols
EditorsF. Cvrcková, V. Zárský
Place of PublicationHertfordshire
PublisherSpringer
Chapter15
Pages231-238
Number of pages8
Edition2
ISBN (Electronic)9781493994694
ISBN (Print)9781493994687
DOIs
Publication statusPublished - 31 May 2019

Publication series

NameMethods in Molecular Biology
Volume1992
ISSN (Print)1064-3745

Fingerprint

Optical Tweezers
Plant Cells

Keywords

  • Confocal microscope
  • Cytoskeleton
  • Endomembrane system
  • Noninvasive manipulation
  • Optical trap
  • Optical tweezers
  • Plant cell

Cite this

Ketelaar, T., de Ruijter, N., & Niehren, S. (2019). Optical trapping in plant cells. In F. Cvrcková, & V. Zárský (Eds.), Methods in Molecular Biology: Methods and protocols (2 ed., pp. 231-238). (Methods in Molecular Biology; Vol. 1992). Hertfordshire: Springer. https://doi.org/10.1007/978-1-4939-9469-4_15
Ketelaar, Tijs ; de Ruijter, Norbert ; Niehren, Stefan. / Optical trapping in plant cells. Methods in Molecular Biology: Methods and protocols. editor / F. Cvrcková ; V. Zárský. 2. ed. Hertfordshire : Springer, 2019. pp. 231-238 (Methods in Molecular Biology).
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keywords = "Confocal microscope, Cytoskeleton, Endomembrane system, Noninvasive manipulation, Optical trap, Optical tweezers, Plant cell",
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Ketelaar, T, de Ruijter, N & Niehren, S 2019, Optical trapping in plant cells. in F Cvrcková & V Zárský (eds), Methods in Molecular Biology: Methods and protocols. 2 edn, Methods in Molecular Biology, vol. 1992, Springer, Hertfordshire, pp. 231-238. https://doi.org/10.1007/978-1-4939-9469-4_15

Optical trapping in plant cells. / Ketelaar, Tijs; de Ruijter, Norbert; Niehren, Stefan.

Methods in Molecular Biology: Methods and protocols. ed. / F. Cvrcková; V. Zárský. 2. ed. Hertfordshire : Springer, 2019. p. 231-238 (Methods in Molecular Biology; Vol. 1992).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Ketelaar T, de Ruijter N, Niehren S. Optical trapping in plant cells. In Cvrcková F, Zárský V, editors, Methods in Molecular Biology: Methods and protocols. 2 ed. Hertfordshire: Springer. 2019. p. 231-238. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9469-4_15