Optical trapping in plant cells

Research output: Chapter in Book/Report/Conference proceedingChapter

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Abstract

Optical tweezers allow noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi-)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope. Next, we describe some working protocols to trap intracellular structures in plant cells
Original languageEnglish
Title of host publicationPlant Cell Morphogenesis
Subtitle of host publicationMethods and Protocols
EditorsV. Zárský, F. Cvrçková
Place of PublicationHertfordshire
PublisherHumana Press
Chapter22
Pages259-265
Volume1080
ISBN (Electronic)9781627036436
ISBN (Print)9781627036429
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in molecular biology
PublisherSpringer Verlag
ISSN (Print)1064-3745

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  • Cite this

    Ketelaar, T., de Ruijter, N. C. A., & Niehren, S. (2014). Optical trapping in plant cells. In V. Zárský, & F. Cvrçková (Eds.), Plant Cell Morphogenesis: Methods and Protocols (Vol. 1080, pp. 259-265). (Methods in molecular biology). Humana Press. https://doi.org/10.1007/978-1-62703-643-6_22