Online and in situ analysis of organs-on-a-chip

Research output: Contribution to journalReview articleAcademicpeer-review

Abstract

Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sensors or online coupling to analytical equipment becomes feasible. Apart from the challenge to detect drug uptake and chemical changes in real-time with high resolution at the microscale, the biggest challenge, is detection of the analyte of interest in cell culture medium, as this contains high amounts of salts, sugars and proteins required by the living cells. In this review online and in situ analytical techniques integrated with organ-on-a-chip devices are discussed with special emphasis on maintaining the biological relevance, achieving analytical compatibility, system integration and final applicability.

LanguageEnglish
Pages138-146
Number of pages9
JournalTrAC - Trends in Analytical Chemistry
Volume115
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Cell culture
Metabolism
Sugars
Culture Media
Salts
Cells
Sensors
Liquids
Pharmaceutical Preparations
Proteins

Keywords

  • Electrochemical sensor
  • Mass spectrometry
  • Online analysis
  • Optical detector
  • Organ-on-a-chip

Cite this

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title = "Online and in situ analysis of organs-on-a-chip",
abstract = "Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sensors or online coupling to analytical equipment becomes feasible. Apart from the challenge to detect drug uptake and chemical changes in real-time with high resolution at the microscale, the biggest challenge, is detection of the analyte of interest in cell culture medium, as this contains high amounts of salts, sugars and proteins required by the living cells. In this review online and in situ analytical techniques integrated with organ-on-a-chip devices are discussed with special emphasis on maintaining the biological relevance, achieving analytical compatibility, system integration and final applicability.",
keywords = "Electrochemical sensor, Mass spectrometry, Online analysis, Optical detector, Organ-on-a-chip",
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pages = "138--146",
journal = "TrAC : Trends in Analytical Chemistry",
issn = "0165-9936",
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Online and in situ analysis of organs-on-a-chip. / Santbergen, Milou J.C.; van der Zande, Meike; Bouwmeester, Hans; Nielen, Michel W.F.

In: TrAC - Trends in Analytical Chemistry, Vol. 115, 01.06.2019, p. 138-146.

Research output: Contribution to journalReview articleAcademicpeer-review

TY - JOUR

T1 - Online and in situ analysis of organs-on-a-chip

AU - Santbergen, Milou J.C.

AU - van der Zande, Meike

AU - Bouwmeester, Hans

AU - Nielen, Michel W.F.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sensors or online coupling to analytical equipment becomes feasible. Apart from the challenge to detect drug uptake and chemical changes in real-time with high resolution at the microscale, the biggest challenge, is detection of the analyte of interest in cell culture medium, as this contains high amounts of salts, sugars and proteins required by the living cells. In this review online and in situ analytical techniques integrated with organ-on-a-chip devices are discussed with special emphasis on maintaining the biological relevance, achieving analytical compatibility, system integration and final applicability.

AB - Organ-on-a-chip technology is used to study biological processes that involve multiple cell types and temporal changes like, homeostasis, metabolism and responses to chemical triggers. Main benefits of organ-on-a-chip systems include: improved mimicking of the in vivo situation, easy manipulation of the microenvironment and low reagent consumption. Exploiting the unique dynamic aspects of organ-on-a-chip technology, such as liquid flow, automated online measurement of parameters by sensors or online coupling to analytical equipment becomes feasible. Apart from the challenge to detect drug uptake and chemical changes in real-time with high resolution at the microscale, the biggest challenge, is detection of the analyte of interest in cell culture medium, as this contains high amounts of salts, sugars and proteins required by the living cells. In this review online and in situ analytical techniques integrated with organ-on-a-chip devices are discussed with special emphasis on maintaining the biological relevance, achieving analytical compatibility, system integration and final applicability.

KW - Electrochemical sensor

KW - Mass spectrometry

KW - Online analysis

KW - Optical detector

KW - Organ-on-a-chip

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JO - TrAC : Trends in Analytical Chemistry

T2 - TrAC : Trends in Analytical Chemistry

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SN - 0165-9936

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