One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages

J. Rubert; N. Sebastià; J.M. Soriano; C. Soler; J. Mañes

doi:10.1016/j.foodchem.2011.01.016

One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages

J. Rubert, N. Sebastià, J.M. Soriano, C. Soler^*, J. Mañes

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

34 Citations (Scopus)

Abstract

A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB₁, AFB₂, AFG₁ and AFG ₂) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C₁₈, while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB₁, AFB₂ and AFG₂.

Original language	English
Pages (from-to)	822-826
Number of pages	5
Journal	Food Chemistry
Volume	127
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2011.01.016
Publication status	Published - 15 Jul 2011
Externally published	Yes

Keywords

Horchata
Monitoring
Mycotoxins
Tiger nuts

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10.1016/j.foodchem.2011.01.016

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title = "One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages",

abstract = "A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB1, AFB2, AFG1 and AFG 2) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C18, while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB1, AFB2 and AFG2.",

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author = "J. Rubert and N. Sebasti{\`a} and J.M. Soriano and C. Soler and J. Ma{\~n}es",

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doi = "10.1016/j.foodchem.2011.01.016",

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T1 - One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages

AU - Rubert, J.

AU - Sebastià, N.

AU - Soriano, J.M.

AU - Soler, C.

AU - Mañes, J.

PY - 2011/7/15

Y1 - 2011/7/15

N2 - A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB1, AFB2, AFG1 and AFG 2) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C18, while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB1, AFB2 and AFG2.

AB - A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB1, AFB2, AFG1 and AFG 2) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C18, while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB1, AFB2 and AFG2.

KW - Horchata

KW - Monitoring

KW - Mycotoxins

KW - Tiger nuts

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DO - 10.1016/j.foodchem.2011.01.016

M3 - Article

AN - SCOPUS:79952311008

SN - 0308-8146

VL - 127

SP - 822

EP - 826

JO - Food Chemistry

JF - Food Chemistry

IS - 2

ER -

One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages

Abstract

Keywords

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