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Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many of these compounds. Yet, environmental influences on the sponge-associated microbiota and bioactive compound production remain elusive. Here, we investigated the changes of microbiota and metabolomes in sponges along a depth range of 1232 m. Using 16S rRNA gene amplicon sequencing and untargeted metabolomics, we assessed prokaryotic and chemical diversities in three deep-sea sponge species: Geodia barretti, Stryphnus fortis, and Weberella bursa. Both prokaryotic communities and metabolome varied significantly with depth, which we hypothesized to be the effect of different water masses. Up to 35.5% of microbial ASVs (amplicon sequence variants) showed significant changes with depth while phylum-level composition of host microbiome remained unchanged. The metabolome varied with depth, with relative quantities of known bioactive compounds increasing or decreasing strongly. Other metabolites varying with depth were compatible solutes regulating osmolarity of the cells. Correlations between prokaryotic community and the bioactive compounds in G. barretti suggested members of Acidobacteria, Proteobacteria, Chloroflexi, or an unclassified prokaryote as potential producers.
|Publication status||Published - 1 Mar 2022|
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Collection data, 16S microbiome and metabolome of Geodia barretti, Stryphnus fortis and Weberella bursa, in the Davis Strait
Steffen, K. (Creator), Indraningrat, A. A. G. (Creator), Erngren, I. (Creator), Haglöf, J. (Creator), Becking, L. (Creator), Kenchington, E. (Creator), Smidt, H. (Creator), Yashayaev, I. (Creator), Pettersson, C. (Creator), Cárdenas, P. (Creator) & Sipkema, D. (Creator), Wageningen University & Research, 2019
Microbiota composition and metabolomic profiles of the deep sea sponges Weberella bursa, Stryphnus fortis and Geodia barretti along a depth gradient
Steffen, K. (Creator), Indraningrat, A. (Creator), Erngren, I. (Creator), Haglöf, J. (Creator), Becking, L. (Creator), Smidt, H. (Creator), Yashayaev, I. (Creator), Kenchington, E. (Creator), Pettersson, C. (Creator), Cárdenas, P. (Creator) & Sipkema, D. (Creator), Wageningen University, 25 Apr 2018
- 1 Finished
SponGES: Deep-sea Sponge Grounds Ecosystems of the North Atlantic: an integrated approach towards their preservation and sustainable exploitation
1/03/16 → 31/12/20
Project: EU research project