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CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might confer risks when present in gene-edited food crops. Through an extensive literature review we gathered information on CRISPR-Cas off-target edits in plants. Most observed off-target changes were small insertions or deletions (1-22 bp) or nucleotide substitutions, and large deletions (>100 bp) were rare. One study detected the insertion of vector-derived DNA sequences, which is important considering the risk assessment of gene-edited plants. Off-target sites had few mismatches (1-3 nt) with the target sequence and were mainly located in protein-coding regions, often in target gene homologues. Off-targets edits were predominantly detected via biased analysis of predicted off-target sites instead of unbiased genome-wide analysis. CRISPR-Cas-edited plants showed lower off-target mutation frequencies than conventionally bred plants. This Review can aid discussions on the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants.
|Journal||ACS Agricultural Science and Technology|
|Publication status||Published - 18 Apr 2022|
- genome editing
- off-target modifications
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- 2 Finished
Risico-analyse aspecten van nieuwe veredelingstechnieken, inclusief synthetische biologie (KB-37-002-013, KB-23-002-027)
1/01/16 → 31/12/21
Beter scoren door innovatie en opschaling kringlooplandbouw (KB-14-001-037, KB-14-002-013)
3/01/11 → 31/12/15