Occurrence and characterization of methicillin resistant Staphylococcus aureus in processed raw foods and ready-to-eat foods in an urban setting of a developing country

Mohammad Aminul Islam, Sahana Parveen, Mahdia Rahman, Mohsina Huq, Ashikun Nabi, Zahed Uddin Mahmood Khan, Niyaz Ahmed, Jaap A. Wagenaar

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Infections by methicillin-resistant Staphylococcus aureus (MRSA) are gradually increasing in the community. In this study, we investigated a total of 162 food samples including 112 ready-to-eat (RTE) foods and 40 processed raw meat and fish samples collected from retail vendors in Dhaka, Bangladesh and determined the occurrence of toxigenic S. aureus and MRSA. Around 22% of samples were positive for S. aureus, RTE foods being more positive (23%) than the processed raw meat/fish samples (18%). Among 35 S. aureus isolates, 74% were resistant to erythromycin, 49% to ciprofloxacin and around 30% to oxacillin and cefoxitin. Around 37% of isolates were resistant to ≥3 classes of antibiotics and 26% of isolates (n = 9) were identified as MRSA. Majority of the isolates were positive for enterotoxin genes (74%), followed by pvl gene (71%), toxic shock syndrome toxin (tsst) gene (17%) and exfoliative toxin genes (11%). Multi locus sequence typing (MLST) of 9 MRSA isolates identified four different types such as ST80 (n = 3), ST6 (n = 2), ST239 (n = 2) and ST361 (n = 2). spa typing of MRSA isolates revealed seven different types including t1198 (n = 2), t315 (n = 2), t037 (n = 1), t275 (n = 1), t304 (n = 1), t8731 (n = 1) and t10546 (n = 1). To our knowledge, this is the first report entailing baseline data on the occurrence of MRSA in RTE foods in Dhaka highlighting a potential public health risk to street food consumers.

LanguageEnglish
Article number503
JournalFrontiers in Microbiology
Volume10
Issue numberMAR
DOIs
Publication statusPublished - 14 Mar 2019

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Developing Countries
Food
Staphylococcus aureus
Meat
Genes
Fishes
Exfoliatins
Cefoxitin
Oxacillin
Bangladesh
Enterotoxins
Erythromycin
Ciprofloxacin
Septic Shock
Raw Foods
Public Health
Anti-Bacterial Agents
Infection

Keywords

  • Methicillin resistant S. aureus
  • MLST
  • Raw meat
  • Ready-to-eat foods
  • Spa typing

Cite this

Islam, Mohammad Aminul ; Parveen, Sahana ; Rahman, Mahdia ; Huq, Mohsina ; Nabi, Ashikun ; Khan, Zahed Uddin Mahmood ; Ahmed, Niyaz ; Wagenaar, Jaap A. / Occurrence and characterization of methicillin resistant Staphylococcus aureus in processed raw foods and ready-to-eat foods in an urban setting of a developing country. In: Frontiers in Microbiology. 2019 ; Vol. 10, No. MAR.
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abstract = "Infections by methicillin-resistant Staphylococcus aureus (MRSA) are gradually increasing in the community. In this study, we investigated a total of 162 food samples including 112 ready-to-eat (RTE) foods and 40 processed raw meat and fish samples collected from retail vendors in Dhaka, Bangladesh and determined the occurrence of toxigenic S. aureus and MRSA. Around 22{\%} of samples were positive for S. aureus, RTE foods being more positive (23{\%}) than the processed raw meat/fish samples (18{\%}). Among 35 S. aureus isolates, 74{\%} were resistant to erythromycin, 49{\%} to ciprofloxacin and around 30{\%} to oxacillin and cefoxitin. Around 37{\%} of isolates were resistant to ≥3 classes of antibiotics and 26{\%} of isolates (n = 9) were identified as MRSA. Majority of the isolates were positive for enterotoxin genes (74{\%}), followed by pvl gene (71{\%}), toxic shock syndrome toxin (tsst) gene (17{\%}) and exfoliative toxin genes (11{\%}). Multi locus sequence typing (MLST) of 9 MRSA isolates identified four different types such as ST80 (n = 3), ST6 (n = 2), ST239 (n = 2) and ST361 (n = 2). spa typing of MRSA isolates revealed seven different types including t1198 (n = 2), t315 (n = 2), t037 (n = 1), t275 (n = 1), t304 (n = 1), t8731 (n = 1) and t10546 (n = 1). To our knowledge, this is the first report entailing baseline data on the occurrence of MRSA in RTE foods in Dhaka highlighting a potential public health risk to street food consumers.",
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Occurrence and characterization of methicillin resistant Staphylococcus aureus in processed raw foods and ready-to-eat foods in an urban setting of a developing country. / Islam, Mohammad Aminul; Parveen, Sahana; Rahman, Mahdia; Huq, Mohsina; Nabi, Ashikun; Khan, Zahed Uddin Mahmood; Ahmed, Niyaz; Wagenaar, Jaap A.

In: Frontiers in Microbiology, Vol. 10, No. MAR, 503, 14.03.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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