Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece

Apostolos Liakopoulos, Jonathan Betts, Roberto La Ragione, Alieda van Essen-Zandbergen, Daniela Ceccarelli, Efthymia Petinaki, Christos K. Koutinas, Dik J. Mevius

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Extended-spectrum cephalosporin-and/or carbapenem-resistant (ESCR and/or CarbR) Enterobacteriaceae constitute a public health hazard because of limited treatment options and are endemic among humans in Greece. Recently, ESCR and CarbR Enterobacteriaceae have been increasingly isolated from companion animals, stressing their potential role as a reservoir for humans. However, the presence of ESCR bacteria in companion animals within Greek households has not been determined yet. Genes conferring the ESCR and CarbR phenotype were detected among canine isolates and their chromosomal or plasmid location was determined. Standard methods were applied for plasmid characterization. The clonal relatedness of the recovered isolates was examined by multilocus sequence typing (MLST). Here, we report the first findings on the presence of ESCR Enterobacteriaceae in healthy Greek dogs. ESCR Escherichia coli isolates were associated with different sequence types (STs), including the human pandemic ST131 clone. The occurrence of human-related ESBL/pAmpC genes, plasmid types and/or strain STS in this animal reservoir suggests possible bilateral transmission.

Original languageEnglish
Article number000768
Pages (from-to)931-935
JournalJournal of Medical Microbiology
Volume67
Issue number7
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Greece
Enterobacteriaceae
Cephalosporins
Dogs
Plasmids
Pets
Multilocus Sequence Typing
Carbapenems
Pandemics
Genes
Canidae
Public Health
Clone Cells
Escherichia coli
Bacteria
Phenotype

Keywords

  • Companion animals
  • E.Coli
  • ESBL
  • K.Pneumoniae
  • MLST
  • Plasmid

Cite this

Liakopoulos, Apostolos ; Betts, Jonathan ; La Ragione, Roberto ; van Essen-Zandbergen, Alieda ; Ceccarelli, Daniela ; Petinaki, Efthymia ; Koutinas, Christos K. ; Mevius, Dik J. / Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece. In: Journal of Medical Microbiology. 2018 ; Vol. 67, No. 7. pp. 931-935.
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Liakopoulos, A, Betts, J, La Ragione, R, van Essen-Zandbergen, A, Ceccarelli, D, Petinaki, E, Koutinas, CK & Mevius, DJ 2018, 'Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece' Journal of Medical Microbiology, vol. 67, no. 7, 000768, pp. 931-935. https://doi.org/10.1099/jmm.0.000768

Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece. / Liakopoulos, Apostolos; Betts, Jonathan; La Ragione, Roberto; van Essen-Zandbergen, Alieda; Ceccarelli, Daniela; Petinaki, Efthymia; Koutinas, Christos K.; Mevius, Dik J.

In: Journal of Medical Microbiology, Vol. 67, No. 7, 000768, 01.07.2018, p. 931-935.

Research output: Contribution to journalArticleAcademicpeer-review

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Liakopoulos A, Betts J, La Ragione R, van Essen-Zandbergen A, Ceccarelli D, Petinaki E et al. Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece. Journal of Medical Microbiology. 2018 Jul 1;67(7):931-935. 000768. https://doi.org/10.1099/jmm.0.000768