Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000

R. van Kranenburg, M. Kleerebezem, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

46 Citations (Scopus)

Abstract

The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.
Original languageEnglish
Pages (from-to)130-136
JournalPlasmid
Volume43
DOIs
Publication statusPublished - 2000

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