Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000

R. van Kranenburg, M. Kleerebezem, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

45 Citations (Scopus)

Abstract

The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.
LanguageEnglish
Pages130-136
JournalPlasmid
Volume43
DOIs
Publication statusPublished - 2000

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Sequence Analysis
Plasmids
Lactobacillus helveticus
Lactococcus lactis
Replicon
DNA Transposable Elements
Multigene Family
Cobalt
Magnesium
Genes

Cite this

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title = "Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000",
abstract = "The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.",
author = "{van Kranenburg}, R. and M. Kleerebezem and {de Vos}, W.M.",
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Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000. / van Kranenburg, R.; Kleerebezem, M.; de Vos, W.M.

In: Plasmid, Vol. 43, 2000, p. 130-136.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000

AU - van Kranenburg, R.

AU - Kleerebezem, M.

AU - de Vos, W.M.

PY - 2000

Y1 - 2000

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AB - The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.

U2 - 10.1006/plas.1999.1453

DO - 10.1006/plas.1999.1453

M3 - Article

VL - 43

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EP - 136

JO - Plasmid

T2 - Plasmid

JF - Plasmid

SN - 0147-619X

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