Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].
|Number of pages||1|
|Journal||Journal of the American Society for Mass Spectrometry|
|Publication status||Published - 1 Dec 2017|
- Forensic toxicology
- Paper spray mass spectrometry
- Whole blood
Teunissen, S. F., Fedick, P. W., Berendsen, B. J. A., Nielen, M. W. F., Eberlin, M. N., Graham Cooks, R., & van Asten, A. C. (2017). Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. Journal of the American Society for Mass Spectrometry, 28(12), 2665-2676. https://doi.org/10.1007/s13361-017-1790-0