Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood

Sebastiaan F. Teunissen, Patrick W. Fedick, Bjorn J.A. Berendsen, Michel W.F. Nielen, Marcos N. Eberlin, R. Graham Cooks, Arian C. van Asten*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].
Original languageEnglish
Pages (from-to)2665-2676
Number of pages1
JournalJournal of the American Society for Mass Spectrometry
Volume28
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • Amphetamines
  • Forensic toxicology
  • Paper spray mass spectrometry
  • Selectivity
  • Validation
  • Whole blood

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