Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood

Sebastiaan F. Teunissen, Patrick W. Fedick, Bjorn J.A. Berendsen, Michel W.F. Nielen, Marcos N. Eberlin, R. Graham Cooks, Arian C. van Asten

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].
LanguageEnglish
Pages2665-2676
Number of pages1
JournalJournal of the American Society for Mass Spectrometry
Volume28
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Amphetamines
Methamphetamine
Toxicology
Mass spectrometry
Mass Spectrometry
3,4-Methylenedioxyamphetamine
Blood
Forensic Toxicology
Amphetamine
Tandem Mass Spectrometry
Limit of Detection
Guidelines
ethylamphetamine
4-fluoroamphetamine
4-methoxyamphetamine

Keywords

  • Amphetamines
  • Forensic toxicology
  • Paper spray mass spectrometry
  • Selectivity
  • Validation
  • Whole blood

Cite this

@article{450b747fa76e4197ba5e214eb2bbf615,
title = "Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood",
abstract = "Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15{\%} and average precision was better than 15{\%}, and better than 20{\%} at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].",
keywords = "Amphetamines, Forensic toxicology, Paper spray mass spectrometry, Selectivity, Validation, Whole blood",
author = "Teunissen, {Sebastiaan F.} and Fedick, {Patrick W.} and Berendsen, {Bjorn J.A.} and Nielen, {Michel W.F.} and Eberlin, {Marcos N.} and {Graham Cooks}, R. and {van Asten}, {Arian C.}",
year = "2017",
month = "12",
day = "1",
doi = "10.1007/s13361-017-1790-0",
language = "English",
volume = "28",
pages = "2665--2676",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1044-0305",
publisher = "Springer Verlag",
number = "12",

}

Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood. / Teunissen, Sebastiaan F.; Fedick, Patrick W.; Berendsen, Bjorn J.A.; Nielen, Michel W.F.; Eberlin, Marcos N.; Graham Cooks, R.; van Asten, Arian C.

In: Journal of the American Society for Mass Spectrometry, Vol. 28, No. 12, 01.12.2017, p. 2665-2676.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood

AU - Teunissen, Sebastiaan F.

AU - Fedick, Patrick W.

AU - Berendsen, Bjorn J.A.

AU - Nielen, Michel W.F.

AU - Eberlin, Marcos N.

AU - Graham Cooks, R.

AU - van Asten, Arian C.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].

AB - Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within ±15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 μL of whole blood. [Figure not available: see fulltext.].

KW - Amphetamines

KW - Forensic toxicology

KW - Paper spray mass spectrometry

KW - Selectivity

KW - Validation

KW - Whole blood

U2 - 10.1007/s13361-017-1790-0

DO - 10.1007/s13361-017-1790-0

M3 - Article

VL - 28

SP - 2665

EP - 2676

JO - Journal of the American Society for Mass Spectrometry

T2 - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1044-0305

IS - 12

ER -