Abstract
A novel cell wall degrading enzyme was identi¿ ed
as a part of cDNA-AFLP project that compared gene
expression pro¿ les in ¿ ve different stages of Globodera
rostochiensis. One Transcript Derived Fragment (TDF),
predominantly expressed in infective second stage
juveniles, encoded a partial open reading frame showing
high similarity to bacterial and fungal pectate lyases
from the polysaccharide lyase 3 family (EC 4.2.2.2).
The corresponding full-length cDNA designated Gr-Pel2
harbours an open reading frame of 759 bp, encoding
polypeptide with molecular mass 27 kDa. Gr-Pel2 is
signi¿ cantly different from previously identi¿ ed Gr-
Pel1 (30% identities and 46% similarities). Sequence
was predicted to have signal peptide for secretion at
its N-terminal end. Digoxigenin-labelled DNA probe
hybridised speci¿ cally to the subventral pharyngeal
glands, which are responsible for secreting all currently
known cell wall degrading enzymes. Heterologous
expression of Gr-Pel2 in Pichia pastoris is in progress, in
order to assess its biochemical properties. Identi¿ cation
of another pectate lyase gene shows the complexity of the
pectinolytic system in nematode secretions.
| Original language | English |
|---|---|
| Title of host publication | Nematology Symposium abstracts |
| Place of Publication | Leiden |
| Publisher | Brill |
| Pages | 222 (283) |
| Volume | 4 |
| Publication status | Published - 2002 |