Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora

Manuel Ferrer*, Olga V. Golyshina, Tatyana N. Chernikova, Amit N. Khachane, Dolores Reyes-Duarte, Vitor A.P. Martins Dos Santos, Carsten Strompl, Kieran Elborough, Graeme Jarvis, Alexander Neef, Michail M. Yakimov, Kenneth N. Timmis, Peter N. Golyshin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

217 Citations (Scopus)

Abstract

A metagenome expression library of bulk DNA extracted from the rumen content of a dairy cow was established in a phage lambda vector and activity-based screening employed to explore the functional diversity of the microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-β-1,4-glucanases and one cyclodextrinase) were identified in the library and characterized. Sequence analysis of the retrieved enzymes revealed that eight (36%) were entirely new and formed deep-branched phylogenetic lineages with no close relatives among known ester- and glycosyl-hydrolases. Bioinformatic analyses of the hydrolase gene sequences, and the sequences and contexts of neighbouring genes, suggested tentative phylogenetic assignments of the rumen organisms producing the retrieved enzymes. The phylogenetic novelty of the hydrolases suggests that some of them may have potential for new applications in biocatalysis.

Original languageEnglish
Pages (from-to)1996-2010
Number of pages15
JournalEnvironmental Microbiology
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

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