TY - JOUR
T1 - Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora
AU - Ferrer, Manuel
AU - Golyshina, Olga V.
AU - Chernikova, Tatyana N.
AU - Khachane, Amit N.
AU - Reyes-Duarte, Dolores
AU - Martins Dos Santos, Vitor A.P.
AU - Strompl, Carsten
AU - Elborough, Kieran
AU - Jarvis, Graeme
AU - Neef, Alexander
AU - Yakimov, Michail M.
AU - Timmis, Kenneth N.
AU - Golyshin, Peter N.
PY - 2005/12
Y1 - 2005/12
N2 - A metagenome expression library of bulk DNA extracted from the rumen content of a dairy cow was established in a phage lambda vector and activity-based screening employed to explore the functional diversity of the microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-β-1,4-glucanases and one cyclodextrinase) were identified in the library and characterized. Sequence analysis of the retrieved enzymes revealed that eight (36%) were entirely new and formed deep-branched phylogenetic lineages with no close relatives among known ester- and glycosyl-hydrolases. Bioinformatic analyses of the hydrolase gene sequences, and the sequences and contexts of neighbouring genes, suggested tentative phylogenetic assignments of the rumen organisms producing the retrieved enzymes. The phylogenetic novelty of the hydrolases suggests that some of them may have potential for new applications in biocatalysis.
AB - A metagenome expression library of bulk DNA extracted from the rumen content of a dairy cow was established in a phage lambda vector and activity-based screening employed to explore the functional diversity of the microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-β-1,4-glucanases and one cyclodextrinase) were identified in the library and characterized. Sequence analysis of the retrieved enzymes revealed that eight (36%) were entirely new and formed deep-branched phylogenetic lineages with no close relatives among known ester- and glycosyl-hydrolases. Bioinformatic analyses of the hydrolase gene sequences, and the sequences and contexts of neighbouring genes, suggested tentative phylogenetic assignments of the rumen organisms producing the retrieved enzymes. The phylogenetic novelty of the hydrolases suggests that some of them may have potential for new applications in biocatalysis.
U2 - 10.1111/j.1462-2920.2005.00920.x
DO - 10.1111/j.1462-2920.2005.00920.x
M3 - Article
C2 - 16309396
AN - SCOPUS:28644438257
SN - 1462-2912
VL - 7
SP - 1996
EP - 2010
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 12
ER -