Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery

Sara Cantera, Irene Sánchez-Andrea, Lidia J. Sadornil, Pedro A. García-Encina, Alfons J.M. Stams, Raúl Muñoz

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6% NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6% NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production.

Original languageEnglish
Pages (from-to)1091-1099
JournalJournal of Environmental Management
Volume231
DOIs
Publication statusPublished - 1 Feb 2019

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Bacteria
Methane
methane
bacterium
Bioconversion
Carbon
carbon
Metabolism
competitiveness
Feedstocks
metabolism
market
refinery
energy source

Keywords

  • Alishewanella
  • CH bio-refinery
  • Ectoine
  • Halomonas
  • Methane treatment

Cite this

Cantera, Sara ; Sánchez-Andrea, Irene ; Sadornil, Lidia J. ; García-Encina, Pedro A. ; Stams, Alfons J.M. ; Muñoz, Raúl. / Novel haloalkaliphilic methanotrophic bacteria : An attempt for enhancing methane bio-refinery. In: Journal of Environmental Management. 2019 ; Vol. 231. pp. 1091-1099.
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abstract = "Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6{\%} NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6{\%} NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production.",
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Novel haloalkaliphilic methanotrophic bacteria : An attempt for enhancing methane bio-refinery. / Cantera, Sara; Sánchez-Andrea, Irene; Sadornil, Lidia J.; García-Encina, Pedro A.; Stams, Alfons J.M.; Muñoz, Raúl.

In: Journal of Environmental Management, Vol. 231, 01.02.2019, p. 1091-1099.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Cantera, Sara

AU - Sánchez-Andrea, Irene

AU - Sadornil, Lidia J.

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KW - Halomonas

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