Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis

S.W. van Es, S.R. Silveira, D.I. Rocha, A. Bimbo, A.P. Martinelli, M.C. Dornelas, G.C. Angenent, G.H. Immink

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The flowers of most dicotyledons have petals that, together with the sepals, initially protect the reproductive organs. Later during development petals are required to open the flower and to attract pollinators. This diverse set of functions demands tight temporal and spatial regulation of petal development. We studied the functioning of the Arabidopsis thaliana TCP5-like transcription factors (TFs) in petals. Overexpression of TCP5 in petal epidermal cells results in smaller petals, whereas tcp5 tcp13 tcp17 triple knockout lines have
wider petals with an increased surface area. Comprehensive expression studies revealed effects of TCP5-like TFs on the expression of genes related to the cell cycle, growth regulation and organ growth. Additionally, the ethylene biosynthesis genes 1-amino-cyclopropane-1-carboxylate (ACC) synthase 2 (ACS2) and ACC oxidase 2 (ACO2) and several ETHYLENE RESPONSE FACTORS (ERFs) are found to be differentially expressed in TCP5 mutant and overexpression lines. Chromatin immunoprecipitation–quantitative PCR showed direct binding of TCP5 to the ACS2 locus in vivo. Ethylene is known to influence cell elongation, and the petal phenotype of the tcp5 tcp13 tcp17 mutant could be complemented by treatment of the plants with an ethylene pathway inhibitor. Taken together, this reveals a novel role for TCP5-like TFs in the regulation of ethylenemediated petal development and growth.
Original languageEnglish
Pages (from-to)867-879
JournalThe Plant Journal
Volume94
Issue number5
Early online date25 Mar 2018
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Arabidopsis
ethylene production
corolla
Transcription Factors
transcription factors
1-aminocyclopropanecarboxylate synthase
Growth
Growth and Development
Chromatin
Cell Cycle
Oxidoreductases
ethylene
Phenotype
Gene Expression
Polymerase Chain Reaction
demand functions
flowers
1-aminocyclopropane-1-carboxylate synthase
mutants
Genes

Cite this

van Es, S. W., Silveira, S. R., Rocha, D. I., Bimbo, A., Martinelli, A. P., Dornelas, M. C., ... Immink, G. H. (2018). Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis. The Plant Journal, 94(5), 867-879. https://doi.org/10.1111/tpj.13904
van Es, S.W. ; Silveira, S.R. ; Rocha, D.I. ; Bimbo, A. ; Martinelli, A.P. ; Dornelas, M.C. ; Angenent, G.C. ; Immink, G.H. / Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis. In: The Plant Journal. 2018 ; Vol. 94, No. 5. pp. 867-879.
@article{2f846c3e9f4e4999a67890740ec77817,
title = "Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis",
abstract = "The flowers of most dicotyledons have petals that, together with the sepals, initially protect the reproductive organs. Later during development petals are required to open the flower and to attract pollinators. This diverse set of functions demands tight temporal and spatial regulation of petal development. We studied the functioning of the Arabidopsis thaliana TCP5-like transcription factors (TFs) in petals. Overexpression of TCP5 in petal epidermal cells results in smaller petals, whereas tcp5 tcp13 tcp17 triple knockout lines havewider petals with an increased surface area. Comprehensive expression studies revealed effects of TCP5-like TFs on the expression of genes related to the cell cycle, growth regulation and organ growth. Additionally, the ethylene biosynthesis genes 1-amino-cyclopropane-1-carboxylate (ACC) synthase 2 (ACS2) and ACC oxidase 2 (ACO2) and several ETHYLENE RESPONSE FACTORS (ERFs) are found to be differentially expressed in TCP5 mutant and overexpression lines. Chromatin immunoprecipitation–quantitative PCR showed direct binding of TCP5 to the ACS2 locus in vivo. Ethylene is known to influence cell elongation, and the petal phenotype of the tcp5 tcp13 tcp17 mutant could be complemented by treatment of the plants with an ethylene pathway inhibitor. Taken together, this reveals a novel role for TCP5-like TFs in the regulation of ethylenemediated petal development and growth.",
author = "{van Es}, S.W. and S.R. Silveira and D.I. Rocha and A. Bimbo and A.P. Martinelli and M.C. Dornelas and G.C. Angenent and G.H. Immink",
year = "2018",
month = "6",
doi = "10.1111/tpj.13904",
language = "English",
volume = "94",
pages = "867--879",
journal = "The Plant Journal",
issn = "0960-7412",
publisher = "Wiley",
number = "5",

}

Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis. / van Es, S.W.; Silveira, S.R.; Rocha, D.I.; Bimbo, A.; Martinelli, A.P.; Dornelas, M.C.; Angenent, G.C.; Immink, G.H.

In: The Plant Journal, Vol. 94, No. 5, 06.2018, p. 867-879.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis

AU - van Es, S.W.

AU - Silveira, S.R.

AU - Rocha, D.I.

AU - Bimbo, A.

AU - Martinelli, A.P.

AU - Dornelas, M.C.

AU - Angenent, G.C.

AU - Immink, G.H.

PY - 2018/6

Y1 - 2018/6

N2 - The flowers of most dicotyledons have petals that, together with the sepals, initially protect the reproductive organs. Later during development petals are required to open the flower and to attract pollinators. This diverse set of functions demands tight temporal and spatial regulation of petal development. We studied the functioning of the Arabidopsis thaliana TCP5-like transcription factors (TFs) in petals. Overexpression of TCP5 in petal epidermal cells results in smaller petals, whereas tcp5 tcp13 tcp17 triple knockout lines havewider petals with an increased surface area. Comprehensive expression studies revealed effects of TCP5-like TFs on the expression of genes related to the cell cycle, growth regulation and organ growth. Additionally, the ethylene biosynthesis genes 1-amino-cyclopropane-1-carboxylate (ACC) synthase 2 (ACS2) and ACC oxidase 2 (ACO2) and several ETHYLENE RESPONSE FACTORS (ERFs) are found to be differentially expressed in TCP5 mutant and overexpression lines. Chromatin immunoprecipitation–quantitative PCR showed direct binding of TCP5 to the ACS2 locus in vivo. Ethylene is known to influence cell elongation, and the petal phenotype of the tcp5 tcp13 tcp17 mutant could be complemented by treatment of the plants with an ethylene pathway inhibitor. Taken together, this reveals a novel role for TCP5-like TFs in the regulation of ethylenemediated petal development and growth.

AB - The flowers of most dicotyledons have petals that, together with the sepals, initially protect the reproductive organs. Later during development petals are required to open the flower and to attract pollinators. This diverse set of functions demands tight temporal and spatial regulation of petal development. We studied the functioning of the Arabidopsis thaliana TCP5-like transcription factors (TFs) in petals. Overexpression of TCP5 in petal epidermal cells results in smaller petals, whereas tcp5 tcp13 tcp17 triple knockout lines havewider petals with an increased surface area. Comprehensive expression studies revealed effects of TCP5-like TFs on the expression of genes related to the cell cycle, growth regulation and organ growth. Additionally, the ethylene biosynthesis genes 1-amino-cyclopropane-1-carboxylate (ACC) synthase 2 (ACS2) and ACC oxidase 2 (ACO2) and several ETHYLENE RESPONSE FACTORS (ERFs) are found to be differentially expressed in TCP5 mutant and overexpression lines. Chromatin immunoprecipitation–quantitative PCR showed direct binding of TCP5 to the ACS2 locus in vivo. Ethylene is known to influence cell elongation, and the petal phenotype of the tcp5 tcp13 tcp17 mutant could be complemented by treatment of the plants with an ethylene pathway inhibitor. Taken together, this reveals a novel role for TCP5-like TFs in the regulation of ethylenemediated petal development and growth.

U2 - 10.1111/tpj.13904

DO - 10.1111/tpj.13904

M3 - Article

VL - 94

SP - 867

EP - 879

JO - The Plant Journal

JF - The Plant Journal

SN - 0960-7412

IS - 5

ER -

van Es SW, Silveira SR, Rocha DI, Bimbo A, Martinelli AP, Dornelas MC et al. Novel functions of the Arabidopsis transcription factor TCP5 in petal development and ethylene biosynthesis. The Plant Journal. 2018 Jun;94(5):867-879. https://doi.org/10.1111/tpj.13904