Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

S.A. Kaba, A.J. Musoke, D. Schaap, T. Schetters, J. Rowlands, A.J. Vermeulen, V. Nene, J.M. Vlak, M.M. van Oers

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products
Original languageEnglish
Pages (from-to)2791-2800
JournalVaccine
Volume23
Issue number21
DOIs
Publication statusPublished - 2005

Keywords

  • theileria-parva sporozoites
  • neutralizing epitopes
  • monoclonal-antibody
  • surface-antigen
  • protection
  • protein
  • stocks
  • immunogenicity
  • immunization
  • leukocytes

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