Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

S.A. Kaba; A.J. Musoke; D. Schaap; T. Schetters; J. Rowlands; A.J. Vermeulen; V. Nene; J.M. Vlak; M.M. van Oers

doi:10.1016/j.vaccine.2004.10.043

Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

S.A. Kaba, A.J. Musoke, D. Schaap, T. Schetters, J. Rowlands, A.J. Vermeulen, V. Nene, J.M. Vlak, M.M. van Oers

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products

Original language	English
Pages (from-to)	2791-2800
Journal	Vaccine
Volume	23
Issue number	21
DOIs	https://doi.org/10.1016/j.vaccine.2004.10.043
Publication status	Published - 2005

Keywords

theileria-parva sporozoites
neutralizing epitopes
monoclonal-antibody
surface-antigen
protection
protein
stocks
immunogenicity
immunization
leukocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2004.10.043

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title = "Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle",

abstract = "Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products",

keywords = "theileria-parva sporozoites, neutralizing epitopes, monoclonal-antibody, surface-antigen, protection, protein, stocks, immunogenicity, immunization, leukocytes",

author = "S.A. Kaba and A.J. Musoke and D. Schaap and T. Schetters and J. Rowlands and A.J. Vermeulen and V. Nene and J.M. Vlak and {van Oers}, M.M.",

year = "2005",

doi = "10.1016/j.vaccine.2004.10.043",

language = "English",

volume = "23",

pages = "2791--2800",

journal = "Vaccine",

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TY - JOUR

T1 - Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

AU - Kaba, S.A.

AU - Musoke, A.J.

AU - Schaap, D.

AU - Schetters, T.

AU - Rowlands, J.

AU - Vermeulen, A.J.

AU - Nene, V.

AU - Vlak, J.M.

AU - van Oers, M.M.

PY - 2005

Y1 - 2005

N2 - Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products

AB - Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67¿SS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67¿SS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100 ¿g per dose, which is a major improvement over previous recombinant p67 products

KW - theileria-parva sporozoites

KW - neutralizing epitopes

KW - monoclonal-antibody

KW - surface-antigen

KW - protection

KW - protein

KW - stocks

KW - immunogenicity

KW - immunization

KW - leukocytes

U2 - 10.1016/j.vaccine.2004.10.043

DO - 10.1016/j.vaccine.2004.10.043

M3 - Article

SN - 0264-410X

VL - 23

SP - 2791

EP - 2800

JO - Vaccine

JF - Vaccine

IS - 21

ER -

Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

Abstract

Keywords

UN SDGs

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