Not sequentially but simultaneously: Facile extraction of proteins and oleosomes from oilseeds

Eleni Ntone, Johannes H. Bitter, Constantinos V. Nikiforidis*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)


Oilseeds represent a sustainable source of oils and proteins that can replace those of animal origin. However, the extraction of oil and proteins from oilseeds currently requires multiple steps and is plagued by undesired reactions occurring during the extraction, which limits valorization. In this paper, we describe a successful method for the simple simultaneous extraction of proteins and oil (as intact oleosomes). Non-defatted dehulled rapeseeds served as oilseed model. First, an aqueous extraction step at pH 9.0 was performed resulting in a protein-oleosome extract, with extraction yields of 78.8 ±0.2 wt% and 82.8 ±0.4 wt% of proteins and oleosomes respectively. Further separation resulted in a protein-rich and an oleosome-rich mixture. The oleosomes were recovered as high oil volume oil-in-water emulsion, while simple filtration of the protein-rich mixture led to a highly soluble (81.4 ±1.9 wt%) protein concentrate. Following this extraction method, complexation between proteins and phenolic compounds was prevented, a clear advantage over the existing methods. These findings emphasize the importance of designing new processes for the extraction of oilseed proteins and oleosomes that could initiate their use in food systems.

Original languageEnglish
Article number105598
JournalFood Hydrocolloids
Publication statusPublished - 1 May 2020


  • Emulsifiers
  • Oil bodies
  • Oil-in-water systems
  • Oleosomes
  • Plant proteins
  • Rapeseed proteins

Fingerprint Dive into the research topics of 'Not sequentially but simultaneously: Facile extraction of proteins and oleosomes from oilseeds'. Together they form a unique fingerprint.

Cite this