Non-isothermal cephalexin hydrolysis by penicillin G acylase immobilized on grafted nylon membranes

M.S. Mohy Eldin, M. Santucci, S. Rossi, J. Tramper, A.E.M. Janssen, C.G.P.H. Schroën, D.G. Mita

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    33 Citations (Scopus)


    A new catalytic membrane has been prepared using a nylon membrane grafted by -radiation with methylmethacrylate (MMA) and using hexamethylenediamine (HMDA) as spacer. Penicillin G acylase (PGA) and cephalexin were employed as catalyst and substrate, respectively. Cephalexin hydrolysis was studied in bioreactors operated under isothermal and non-isothermal conditions. A hydrolysis increase was found when the temperature of the warm membrane surface was kept constant and the temperature of the other membrane surface was kept at a lower value. The hydrolysis increase was linearly proportional to the applied temperature difference. Cephalexin hydrolysis increased to about 10 hen a temperature difference of 1°C was applied across the catalytic membrane. These results have been attributed to the non-isothermal cephalexin transport across the membrane, i.e., to the process of thermodialysis. In this way, the enzyme immobilized on and into the membrane reacts with a substrate concentration higher than that produced by simple diffusion under isothermal conditions.
    Original languageEnglish
    Pages (from-to)221-232
    JournalJournal of Molecular Catalysis. B, Enzymatic
    Publication statusPublished - 2000

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