Non-Genetic Generation of Antibody Conjugates Based on Chemoenzymatic Tyrosine Click Chemistry

Jorick J. Bruins, Johannes A.M. Damen, Marloes A. Wijdeven, Lianne P.W.M. Lelieveldt, Floris L. Van Delft, Bauke Albada*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The availability of tools to generate homogeneous and stable antibody conjugates without recombinant DNA technology is a valuable asset in fields spanning from in vitro diagnostics to in vivo imaging and therapeutics. We present here a general approach for the conjugation to human IgG1 antibodies, by employing a straightforward two-stage protocol based on antibody deglycosylation followed by tyrosinase-mediated ortho-quinone strain-promoted click chemistry. The technology is validated by the efficient and clean generation of highly potent DAR2 and DAR4 antibody-drug conjugates (ADCs) with cytotoxic payloads MMAE or PBD dimer, and their in vitro evaluation.

Original languageEnglish
Pages (from-to)2167–2172
JournalBioconjugate Chemistry
Volume32
Issue number10
DOIs
Publication statusPublished - 14 Sep 2021

Fingerprint

Dive into the research topics of 'Non-Genetic Generation of Antibody Conjugates Based on Chemoenzymatic Tyrosine Click Chemistry'. Together they form a unique fingerprint.

Cite this