Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog

Olivier Serralbo, José Manuel Pérez-Pérez, Renze Heidstra, Ben Scheres*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

44 Citations (Scopus)

Abstract

The Arabidopsis HOBBIT (HBT) gene encodes a homolog of the CDC27 anaphase-promoting complex/cyclosome subunit and is essential for postembryonic development. We induced loss-of-function clones by Cre/lox-mediated recombination of a single complementing HBT transgene in a background homozygous for the strong mutant allele hbt2311. Defects in cell division and cell expansion are the primary consequences of ubiquitous postembryonic HBT excision. In roots, both cell division and cell expansion are rapidly affected. In contrast, in leaf primordia, cell division and cell expansion halt after a lag phase, which results in different severities of defects in the proximodistal and mediolateral axes. Surprisingly, small clones reveal non-cell-autonomous rescue of hbt mutant cells, indicating a previously unrecognized compensation mechanism for reduced activity of an anaphase-promoting complex/cyclosome component critical for cell cycle progression.

Original languageEnglish
Pages (from-to)13250-13255
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number35
DOIs
Publication statusPublished - 29 Aug 2006
Externally publishedYes

Keywords

  • Cell cycle
  • Clonal analysis
  • Growth control
  • Root development

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