Nitrogen availability and defense of tomato against two-spotted spider mite

E. Hoffland, M. Dicke, W. van Tintelen, H. Dijkman, M.L. van Beusichem

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

The aim of this work was to study how nitrogen availability affects within-plant allocation to growth and secondary metabolites and how this allocation affects host selection by herbivores. Tomato plants (Lycopersicon esculentum) were grown at six levels of nitrogen availability. When nitrogen availability increased, plant relative growth rate increased, but tissue carbon/nitrogen ratio in the second oldest true leaf and allocation to large glandular trichomes (type VI) as well as to the defense compounds rutin and chlorogenic acid decreased. Leaf protein concentration increased. Two-spotted spider mite (Tetranychus urticae) females responded significantly to these changes: in dual choice tests they preferred leaf disks from plants grown at high nitrogen availability, with a low C/N ratio. This preference persisted in an olfactometer in which the mites were offered only the odors released by leaves with damaged trichomes. We conclude that in a tomato leaf increased nitrogen availability leads to decreased allocation to defenses, and that repellent volatiles released by trichomes play a key role in affecting leaf selection by two-spotted spider mite females.
Original languageEnglish
Pages (from-to)2697-2711
JournalJournal of Chemical Ecology
Volume26
DOIs
Publication statusPublished - 2000

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Tetranychidae
Tetranychus urticae
Lycopersicon esculentum
mite
spider
Nitrogen
Availability
tomatoes
Trichomes
trichome
nitrogen
trichomes
leaves
carbon nitrogen ratio
leaf protein
olfactometers
host selection
Chlorogenic Acid
rutin
repellents

Cite this

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title = "Nitrogen availability and defense of tomato against two-spotted spider mite",
abstract = "The aim of this work was to study how nitrogen availability affects within-plant allocation to growth and secondary metabolites and how this allocation affects host selection by herbivores. Tomato plants (Lycopersicon esculentum) were grown at six levels of nitrogen availability. When nitrogen availability increased, plant relative growth rate increased, but tissue carbon/nitrogen ratio in the second oldest true leaf and allocation to large glandular trichomes (type VI) as well as to the defense compounds rutin and chlorogenic acid decreased. Leaf protein concentration increased. Two-spotted spider mite (Tetranychus urticae) females responded significantly to these changes: in dual choice tests they preferred leaf disks from plants grown at high nitrogen availability, with a low C/N ratio. This preference persisted in an olfactometer in which the mites were offered only the odors released by leaves with damaged trichomes. We conclude that in a tomato leaf increased nitrogen availability leads to decreased allocation to defenses, and that repellent volatiles released by trichomes play a key role in affecting leaf selection by two-spotted spider mite females.",
author = "E. Hoffland and M. Dicke and {van Tintelen}, W. and H. Dijkman and {van Beusichem}, M.L.",
year = "2000",
doi = "10.1023/A:1026477423988",
language = "English",
volume = "26",
pages = "2697--2711",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer Verlag",

}

Nitrogen availability and defense of tomato against two-spotted spider mite. / Hoffland, E.; Dicke, M.; van Tintelen, W.; Dijkman, H.; van Beusichem, M.L.

In: Journal of Chemical Ecology, Vol. 26, 2000, p. 2697-2711.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Nitrogen availability and defense of tomato against two-spotted spider mite

AU - Hoffland, E.

AU - Dicke, M.

AU - van Tintelen, W.

AU - Dijkman, H.

AU - van Beusichem, M.L.

PY - 2000

Y1 - 2000

N2 - The aim of this work was to study how nitrogen availability affects within-plant allocation to growth and secondary metabolites and how this allocation affects host selection by herbivores. Tomato plants (Lycopersicon esculentum) were grown at six levels of nitrogen availability. When nitrogen availability increased, plant relative growth rate increased, but tissue carbon/nitrogen ratio in the second oldest true leaf and allocation to large glandular trichomes (type VI) as well as to the defense compounds rutin and chlorogenic acid decreased. Leaf protein concentration increased. Two-spotted spider mite (Tetranychus urticae) females responded significantly to these changes: in dual choice tests they preferred leaf disks from plants grown at high nitrogen availability, with a low C/N ratio. This preference persisted in an olfactometer in which the mites were offered only the odors released by leaves with damaged trichomes. We conclude that in a tomato leaf increased nitrogen availability leads to decreased allocation to defenses, and that repellent volatiles released by trichomes play a key role in affecting leaf selection by two-spotted spider mite females.

AB - The aim of this work was to study how nitrogen availability affects within-plant allocation to growth and secondary metabolites and how this allocation affects host selection by herbivores. Tomato plants (Lycopersicon esculentum) were grown at six levels of nitrogen availability. When nitrogen availability increased, plant relative growth rate increased, but tissue carbon/nitrogen ratio in the second oldest true leaf and allocation to large glandular trichomes (type VI) as well as to the defense compounds rutin and chlorogenic acid decreased. Leaf protein concentration increased. Two-spotted spider mite (Tetranychus urticae) females responded significantly to these changes: in dual choice tests they preferred leaf disks from plants grown at high nitrogen availability, with a low C/N ratio. This preference persisted in an olfactometer in which the mites were offered only the odors released by leaves with damaged trichomes. We conclude that in a tomato leaf increased nitrogen availability leads to decreased allocation to defenses, and that repellent volatiles released by trichomes play a key role in affecting leaf selection by two-spotted spider mite females.

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DO - 10.1023/A:1026477423988

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JO - Journal of Chemical Ecology

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SN - 0098-0331

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