Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 361-370 |
| Journal | Developmental and Comparative Immunology |
| Volume | 84 |
| DOIs | |
| Publication status | Published - Jul 2018 |
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Keywords
- Dendritic cell
- Innate immunity
- Neonate
- Porcine
- Toll like receptor ligand
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Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation. / Vreman, Sandra; Auray, Gael; Savelkoul, Huub F.J.; Rebel, Annemarie; Summerfield, Artur; Stockhofe-Zurwieden, Norbert.
In: Developmental and Comparative Immunology, Vol. 84, 07.2018, p. 361-370.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation
AU - Vreman, Sandra
AU - Auray, Gael
AU - Savelkoul, Huub F.J.
AU - Rebel, Annemarie
AU - Summerfield, Artur
AU - Stockhofe-Zurwieden, Norbert
PY - 2018/7
Y1 - 2018/7
N2 - The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.
AB - The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.
KW - Dendritic cell
KW - Innate immunity
KW - Neonate
KW - Porcine
KW - Toll like receptor ligand
U2 - 10.1016/j.dci.2018.03.012
DO - 10.1016/j.dci.2018.03.012
M3 - Article
VL - 84
SP - 361
EP - 370
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
ER -