Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.
Original languageEnglish
Pages (from-to)361-370
JournalDevelopmental and Comparative Immunology
Volume84
DOIs
Publication statusPublished - Jul 2018

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Dendritic Cells
Swine
Ligands
Toll-Like Receptor 2
Antigen-Presenting Cells
Innate Immunity
Monocytes
Flow Cytometry
Up-Regulation
Messenger RNA

Keywords

  • Dendritic cell
  • Innate immunity
  • Neonate
  • Porcine
  • Toll like receptor ligand

Cite this

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title = "Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation",
abstract = "The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.",
keywords = "Dendritic cell, Innate immunity, Neonate, Porcine, Toll like receptor ligand",
author = "Sandra Vreman and Gael Auray and Savelkoul, {Huub F.J.} and Annemarie Rebel and Artur Summerfield and Norbert Stockhofe-Zurwieden",
year = "2018",
month = "7",
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language = "English",
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pages = "361--370",
journal = "Developmental and Comparative Immunology",
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Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation. / Vreman, Sandra; Auray, Gael; Savelkoul, Huub F.J.; Rebel, Annemarie; Summerfield, Artur; Stockhofe-Zurwieden, Norbert.

In: Developmental and Comparative Immunology, Vol. 84, 07.2018, p. 361-370.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation

AU - Vreman, Sandra

AU - Auray, Gael

AU - Savelkoul, Huub F.J.

AU - Rebel, Annemarie

AU - Summerfield, Artur

AU - Stockhofe-Zurwieden, Norbert

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N2 - The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.

AB - The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.

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