Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation

Sandra Vreman*, Gael Auray, Huub F.J. Savelkoul, Annemarie Rebel, Artur Summerfield, Norbert Stockhofe-Zurwieden

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.
Original languageEnglish
Pages (from-to)361-370
JournalDevelopmental and Comparative Immunology
Volume84
DOIs
Publication statusPublished - Jul 2018

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Keywords

  • Dendritic cell
  • Innate immunity
  • Neonate
  • Porcine
  • Toll like receptor ligand

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