Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture

N. Askari, G.J. de Klerk

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Plants are characterized by a negative hydrostatic pressure, brought about by transpiration and by capillary activity of xylem vessels (Taiz and Zeiger, 2010). Because of this, a stem that is being cut sucks up what is nearby. Often this is air but it may also be liquid. The diameter of the xylem vessels is 50-100 μm, so when the liquid contains bacteria (that are typically 0.5-5.0 μm), they will enter deeply into the tissue (Askari et al., 2014; De Klerk et al., 2014). To our knowledge, this alleged source of contamination has never been examined.
Original languageEnglish
Title of host publicationProceedings of the 2017 Annual Meeting of the International Plant Propagators' Society
EditorsC.W. Heuser Jr., W.G. Roberts, C. Larkman, S. Gunn, M. Tomita, H. Foulds, F.T. Davies, E. Blythe
PublisherInternational Society for Horticultural Science
Pages85-87
ISBN (Print)9789462612099
DOIs
Publication statusPublished - 5 Oct 2018
EventInternational Plant Propagators' Society 2017 - Alphen aan de Rijn, Netherlands
Duration: 10 Jul 201714 Jul 2017

Publication series

NameActa Horticulturae
Volume1212
ISSN (Print)0567-7572
ISSN (Electronic)2406-6168

Conference

ConferenceInternational Plant Propagators' Society 2017
CountryNetherlands
CityAlphen aan de Rijn
Period10/07/1714/07/17

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    Askari, N., & de Klerk, G. J. (2018). Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture. In C. W. Heuser Jr., W. G. Roberts, C. Larkman, S. Gunn, M. Tomita, H. Foulds, F. T. Davies, & E. Blythe (Eds.), Proceedings of the 2017 Annual Meeting of the International Plant Propagators' Society (pp. 85-87). (Acta Horticulturae; Vol. 1212). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2018.1212.17