Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits-lateral root length and total root length-showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.
LanguageEnglish
Pages292-309
JournalJournal of Integrative Plant Biology
Volume60
Issue number4
Early online date2018
DOIs
Publication statusPublished - Apr 2018

Fingerprint

Plant Growth Regulators
Arabidopsis
hormones
Hormones
plant hormones
Zeatin
Cytokinins
Plant Development
Liquid chromatography
Glucosides
Mass spectrometers
Liquid Chromatography
root systems
ultra-performance liquid chromatography
quantitative genetics
zeatin
spectrometers
quantitative traits
indole acetic acid
cytokinins

Cite this

@article{782ee6671e0548dc98e4262e39bd876e,
title = "Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture",
abstract = "Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits-lateral root length and total root length-showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.",
author = "Sangseok Lee and Sergeeva, {Lidiya I.} and Dick Vreugdenhil",
year = "2018",
month = "4",
doi = "10.1111/jipb.12617",
language = "English",
volume = "60",
pages = "292--309",
journal = "Journal of Integrative Plant Biology",
issn = "1672-9072",
publisher = "Wiley",
number = "4",

}

Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture. / Lee, Sangseok; Sergeeva, Lidiya I.; Vreugdenhil, Dick.

In: Journal of Integrative Plant Biology, Vol. 60, No. 4, 04.2018, p. 292-309.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture

AU - Lee, Sangseok

AU - Sergeeva, Lidiya I.

AU - Vreugdenhil, Dick

PY - 2018/4

Y1 - 2018/4

N2 - Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits-lateral root length and total root length-showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.

AB - Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits-lateral root length and total root length-showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.

U2 - 10.1111/jipb.12617

DO - 10.1111/jipb.12617

M3 - Article

VL - 60

SP - 292

EP - 309

JO - Journal of Integrative Plant Biology

T2 - Journal of Integrative Plant Biology

JF - Journal of Integrative Plant Biology

SN - 1672-9072

IS - 4

ER -