Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes

C.A. Valero Jimenez, A.J.M. Debets, J.A. van Kan, S.E. Schoustra, W. Takken, B.J. Zwaan, C.J.M. Koenraadt

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Background Insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control. Methods Laboratory bioassays were performed on adult female mosquitoes (Anopheles coluzzii) with spores from 29 isolates of B. bassiana, originating from different parts of the world. In addition, phenotypic characteristics of the fungal isolates such as sporulation, spore size and growth rate were studied to explore their relationship with virulence. Results All tested isolates of B. bassiana killed An. coluzzii mosquitoes, and the rate at which this happened differed significantly among the isolates. The risk of mosquitoes dying was around ten times higher when they were exposed to the most virulent as compared to the least virulent isolate. There was significant variation among isolates in spore size, growth rate and sporulation, but none of these morphological characteristics were correlated, and thus predictive, for the ability of the fungal isolate to kill malaria mosquitoes. Conclusions This study shows that there is a wide natural variation in virulence of isolates of B. bassiana, and that selecting an appropriate fungal isolate is highly relevant in killing and thus controlling malaria mosquitoes, particularly if used as part of an integrated vector management strategy. Also, the wide variation observed in virulence offers the opportunity to better understand the molecular and genetic mechanisms that drive this variation and thus to address the potential development of resistance against entomopathogenic fungi
Original languageEnglish
Article number479
Number of pages8
JournalMalaria Journal
Volume13
DOIs
Publication statusPublished - 2014

Fingerprint

Beauveria
Culicidae
Malaria
Virulence
Fungi
Spores
Anopheles
Growth
Biological Assay
Molecular Biology

Keywords

  • metarhizium-anisopliae
  • anopheles-gambiae
  • infection
  • agents
  • susceptibility
  • degradation
  • persistence
  • expression
  • resistance
  • behavior

Cite this

@article{bf747214167f451c8cbdee13eb860bca,
title = "Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes",
abstract = "Background Insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control. Methods Laboratory bioassays were performed on adult female mosquitoes (Anopheles coluzzii) with spores from 29 isolates of B. bassiana, originating from different parts of the world. In addition, phenotypic characteristics of the fungal isolates such as sporulation, spore size and growth rate were studied to explore their relationship with virulence. Results All tested isolates of B. bassiana killed An. coluzzii mosquitoes, and the rate at which this happened differed significantly among the isolates. The risk of mosquitoes dying was around ten times higher when they were exposed to the most virulent as compared to the least virulent isolate. There was significant variation among isolates in spore size, growth rate and sporulation, but none of these morphological characteristics were correlated, and thus predictive, for the ability of the fungal isolate to kill malaria mosquitoes. Conclusions This study shows that there is a wide natural variation in virulence of isolates of B. bassiana, and that selecting an appropriate fungal isolate is highly relevant in killing and thus controlling malaria mosquitoes, particularly if used as part of an integrated vector management strategy. Also, the wide variation observed in virulence offers the opportunity to better understand the molecular and genetic mechanisms that drive this variation and thus to address the potential development of resistance against entomopathogenic fungi",
keywords = "metarhizium-anisopliae, anopheles-gambiae, infection, agents, susceptibility, degradation, persistence, expression, resistance, behavior",
author = "{Valero Jimenez}, C.A. and A.J.M. Debets and {van Kan}, J.A. and S.E. Schoustra and W. Takken and B.J. Zwaan and C.J.M. Koenraadt",
year = "2014",
doi = "10.1186/1475-2875-13-479",
language = "English",
volume = "13",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "Springer Verlag",

}

Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes. / Valero Jimenez, C.A.; Debets, A.J.M.; van Kan, J.A.; Schoustra, S.E.; Takken, W.; Zwaan, B.J.; Koenraadt, C.J.M.

In: Malaria Journal, Vol. 13, 479, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes

AU - Valero Jimenez, C.A.

AU - Debets, A.J.M.

AU - van Kan, J.A.

AU - Schoustra, S.E.

AU - Takken, W.

AU - Zwaan, B.J.

AU - Koenraadt, C.J.M.

PY - 2014

Y1 - 2014

N2 - Background Insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control. Methods Laboratory bioassays were performed on adult female mosquitoes (Anopheles coluzzii) with spores from 29 isolates of B. bassiana, originating from different parts of the world. In addition, phenotypic characteristics of the fungal isolates such as sporulation, spore size and growth rate were studied to explore their relationship with virulence. Results All tested isolates of B. bassiana killed An. coluzzii mosquitoes, and the rate at which this happened differed significantly among the isolates. The risk of mosquitoes dying was around ten times higher when they were exposed to the most virulent as compared to the least virulent isolate. There was significant variation among isolates in spore size, growth rate and sporulation, but none of these morphological characteristics were correlated, and thus predictive, for the ability of the fungal isolate to kill malaria mosquitoes. Conclusions This study shows that there is a wide natural variation in virulence of isolates of B. bassiana, and that selecting an appropriate fungal isolate is highly relevant in killing and thus controlling malaria mosquitoes, particularly if used as part of an integrated vector management strategy. Also, the wide variation observed in virulence offers the opportunity to better understand the molecular and genetic mechanisms that drive this variation and thus to address the potential development of resistance against entomopathogenic fungi

AB - Background Insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control. Methods Laboratory bioassays were performed on adult female mosquitoes (Anopheles coluzzii) with spores from 29 isolates of B. bassiana, originating from different parts of the world. In addition, phenotypic characteristics of the fungal isolates such as sporulation, spore size and growth rate were studied to explore their relationship with virulence. Results All tested isolates of B. bassiana killed An. coluzzii mosquitoes, and the rate at which this happened differed significantly among the isolates. The risk of mosquitoes dying was around ten times higher when they were exposed to the most virulent as compared to the least virulent isolate. There was significant variation among isolates in spore size, growth rate and sporulation, but none of these morphological characteristics were correlated, and thus predictive, for the ability of the fungal isolate to kill malaria mosquitoes. Conclusions This study shows that there is a wide natural variation in virulence of isolates of B. bassiana, and that selecting an appropriate fungal isolate is highly relevant in killing and thus controlling malaria mosquitoes, particularly if used as part of an integrated vector management strategy. Also, the wide variation observed in virulence offers the opportunity to better understand the molecular and genetic mechanisms that drive this variation and thus to address the potential development of resistance against entomopathogenic fungi

KW - metarhizium-anisopliae

KW - anopheles-gambiae

KW - infection

KW - agents

KW - susceptibility

KW - degradation

KW - persistence

KW - expression

KW - resistance

KW - behavior

U2 - 10.1186/1475-2875-13-479

DO - 10.1186/1475-2875-13-479

M3 - Article

VL - 13

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

M1 - 479

ER -