Dietary restriction (DR) is considered a near universal means of lifespan extension in animals. In addition to extending lifespan, DR has been shown to prevent age-related diseases such as cardiovascular disease, diabetes and dementia. DR therefore appears to act generally in keeping organisms relatively healthy until older age. In C. elegans, DR has been investigated in N2 using a wide range of methods and this work has been important in developing our understanding of the mechanisms by which DR extends lifespan. Here, we present an analysis of lifespan under normal conditions (NGM plates and ad libitum food) and under conditions of mild dietary restriction (dauer ager plates and restricted food) for a new panel of 200 sequenced genotyped recombinant inbred lines (RILs) derived from four wild isolates: JU1511, JU1926m JU1931 and JU1941. These strains are representative of genotypically distinct groups of C. elegans isolates from France and are distinct from both N2 and CB4856. The new panel should therefore be useful in determining the genotype-phenotype relationship in the absence of laboratory derived alleles and be more indicative of subtle allelic effects in wild populations. Our analyses show there is extensive variation among the RILs in lifespan under both conditions, suggesting polygenic control. We also show extensive variation in the effect of DR on lifespan of the RILs. Here, the majority of the RILs showed the expected response, with DR prolonging lifespan, however in about 1/3 of the RILs DR reduces lifespan. These data are suggestive of either transgressive segregation or of negative epistasis. Mapping of these data identify multiple candidate QTLs for these traits. Future work aims to locate the causative alleles underlying these QTLs.
|Title of host publication||Proceedings of The 2014 Evolution of Caenorhabditis and Other Nematodes Conference|
|Publication status||Published - 2014|
|Event||The 2014 Evolution of Caenorhabditis and Other Nematodes Conference, Cambridge, UK - |
Duration: 14 Jun 2014 → 17 Jun 2014
|Conference||The 2014 Evolution of Caenorhabditis and Other Nematodes Conference, Cambridge, UK|
|Period||14/06/14 → 17/06/14|