Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans

Alena Kadlecová, Tomáš Jirsa, Ondřej Novák, Jan Kammenga, Miroslav Strnad, Jiří Voller*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)


Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.

Original languageEnglish
Pages (from-to)109-120
Issue number2
Early online date18 Dec 2017
Publication statusPublished - Apr 2018


  • Aging
  • Caenorhabditis elegans
  • Cytokinin
  • Kinetin
  • Phytohormones
  • Topolin
  • Zeatin


Dive into the research topics of 'Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans'. Together they form a unique fingerprint.

Cite this