Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans

Alena Kadlecová, Tomáš Jirsa, Ondřej Novák, Jan Kammenga, Miroslav Strnad, Jiří Voller*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.

Original languageEnglish
Pages (from-to)109-120
JournalBiogerontology
Volume19
Issue number2
Early online date18 Dec 2017
DOIs
Publication statusPublished - Apr 2018

Fingerprint

Kinetin
Cytokinins
Plant Growth Regulators
Caenorhabditis elegans
Oxidative Stress
Zeatin
Somatomedins
Cosmetics
Diptera
Reactive Oxygen Species
Fruit
Fibroblasts
Hot Temperature
High Pressure Liquid Chromatography
Insulin
Growth

Keywords

  • Aging
  • Caenorhabditis elegans
  • Cytokinin
  • Kinetin
  • Phytohormones
  • Topolin
  • Zeatin

Cite this

Kadlecová, Alena ; Jirsa, Tomáš ; Novák, Ondřej ; Kammenga, Jan ; Strnad, Miroslav ; Voller, Jiří. / Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans. In: Biogerontology. 2018 ; Vol. 19, No. 2. pp. 109-120.
@article{dd9440708cfa4088b33a4596dd5dfafd,
title = "Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans",
abstract = "Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.",
keywords = "Aging, Caenorhabditis elegans, Cytokinin, Kinetin, Phytohormones, Topolin, Zeatin",
author = "Alena Kadlecov{\'a} and Tom{\'a}š Jirsa and Ondřej Nov{\'a}k and Jan Kammenga and Miroslav Strnad and Jiř{\'i} Voller",
year = "2018",
month = "4",
doi = "10.1007/s10522-017-9742-4",
language = "English",
volume = "19",
pages = "109--120",
journal = "Biogerontology",
issn = "1389-5729",
publisher = "Springer Verlag",
number = "2",

}

Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans. / Kadlecová, Alena; Jirsa, Tomáš; Novák, Ondřej; Kammenga, Jan; Strnad, Miroslav; Voller, Jiří.

In: Biogerontology, Vol. 19, No. 2, 04.2018, p. 109-120.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans

AU - Kadlecová, Alena

AU - Jirsa, Tomáš

AU - Novák, Ondřej

AU - Kammenga, Jan

AU - Strnad, Miroslav

AU - Voller, Jiří

PY - 2018/4

Y1 - 2018/4

N2 - Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.

AB - Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies’ lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins’ activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5′-monophosphate.

KW - Aging

KW - Caenorhabditis elegans

KW - Cytokinin

KW - Kinetin

KW - Phytohormones

KW - Topolin

KW - Zeatin

U2 - 10.1007/s10522-017-9742-4

DO - 10.1007/s10522-017-9742-4

M3 - Article

VL - 19

SP - 109

EP - 120

JO - Biogerontology

JF - Biogerontology

SN - 1389-5729

IS - 2

ER -