Natural Anti-Gal and Salmonella-Specific Antibodies in Bile and Plasma of Hens Differing in Diet Efficiency

P.F. Cotter, E. van Eerden

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Specific anti-Salmonella enteritidis (SE) and natural anti-¿-gal epitope (Gal¿1-3Galß-1-4GlcNAc-R; anti-Gal) antibodies were measured in plasma sample pools and individual bile specimens obtained from hens differing in diet efficiency. More SE somatic (O) and flagellar (H) antibodies were found in plasma pools from efficient hens (R¿) compared with nonefficient hens (R+) after oral challenge with live SE. Mean titers of somatic agglutinins in bile were 2.3 in R¿ hens and 1.9 in R+ hens (P = 0.06) following live challenge. Salmonella enteritidis antibodies were also found in bile of nonchallenged hens of both types but their levels were not significantly different. Flagellar (H) agglutinin scores were higher in SE-challenged hens compared with nonchallenged hens (3.1 vs. 2.1; P is less than or equal to 0.004) but efficiency types did not differ. Bile also contained high titers of the anti-Gal antibody indicated by the agglutination of glutaraldehyde-stabilized rabbit erythrocytes. The average titer of all SE-nonexposed hens was 9.0 corresponding to 1:5,120 when corrected for the initial dilution and expressed in conventional terms. Salmonella enteritidis exposure was associated with higher anti-Gal titers. The average anti-Gal titer for all SE-exposed hens was 10.0, corresponding to 1:10,240 in conventional terms; this difference was significant (P is less than or equal to 0.016). Diet efficiency type-associated differences in anti-Gal titers were not significant. Collectively, our data indicate that diet efficiency status is not associated with compromised Salmonella-specific immune responses. Rather it appears that the immune responses of diet efficient hens (R¿) are also more efficient. This is because R¿ hens produced higher levels of O- and H-type antibody only as the result of direct exposure to living SE. On the contrary, R+ hens produced H-type antibody because of challenge with heat-killed SE, a circumstance that will not result in disease. Moreover, the hen type difference does not seem to occur at the expense of innate immunity as measured by anti-Gal antibody levels
Original languageEnglish
Pages (from-to)435-440
JournalPoultry Science
Volume85
Issue number3
DOIs
Publication statusPublished - 2006

Fingerprint

bile
hens
Salmonella
Salmonella Enteritidis
antibodies
diet
agglutinins
immune response
glutaraldehyde
agglutination
direct contact
epitopes
mouth
erythrocytes
rabbits

Keywords

  • residual feed-intake
  • growing layer hens
  • enteritidis infection
  • immune-responses
  • selection
  • chickens

Cite this

@article{23aac266b94941c7aa7f6c050299aa90,
title = "Natural Anti-Gal and Salmonella-Specific Antibodies in Bile and Plasma of Hens Differing in Diet Efficiency",
abstract = "Specific anti-Salmonella enteritidis (SE) and natural anti-¿-gal epitope (Gal¿1-3Gal{\ss}-1-4GlcNAc-R; anti-Gal) antibodies were measured in plasma sample pools and individual bile specimens obtained from hens differing in diet efficiency. More SE somatic (O) and flagellar (H) antibodies were found in plasma pools from efficient hens (R¿) compared with nonefficient hens (R+) after oral challenge with live SE. Mean titers of somatic agglutinins in bile were 2.3 in R¿ hens and 1.9 in R+ hens (P = 0.06) following live challenge. Salmonella enteritidis antibodies were also found in bile of nonchallenged hens of both types but their levels were not significantly different. Flagellar (H) agglutinin scores were higher in SE-challenged hens compared with nonchallenged hens (3.1 vs. 2.1; P is less than or equal to 0.004) but efficiency types did not differ. Bile also contained high titers of the anti-Gal antibody indicated by the agglutination of glutaraldehyde-stabilized rabbit erythrocytes. The average titer of all SE-nonexposed hens was 9.0 corresponding to 1:5,120 when corrected for the initial dilution and expressed in conventional terms. Salmonella enteritidis exposure was associated with higher anti-Gal titers. The average anti-Gal titer for all SE-exposed hens was 10.0, corresponding to 1:10,240 in conventional terms; this difference was significant (P is less than or equal to 0.016). Diet efficiency type-associated differences in anti-Gal titers were not significant. Collectively, our data indicate that diet efficiency status is not associated with compromised Salmonella-specific immune responses. Rather it appears that the immune responses of diet efficient hens (R¿) are also more efficient. This is because R¿ hens produced higher levels of O- and H-type antibody only as the result of direct exposure to living SE. On the contrary, R+ hens produced H-type antibody because of challenge with heat-killed SE, a circumstance that will not result in disease. Moreover, the hen type difference does not seem to occur at the expense of innate immunity as measured by anti-Gal antibody levels",
keywords = "residual feed-intake, growing layer hens, enteritidis infection, immune-responses, selection, chickens",
author = "P.F. Cotter and {van Eerden}, E.",
year = "2006",
doi = "10.1093/ps/85.3.435",
language = "English",
volume = "85",
pages = "435--440",
journal = "Poultry Science",
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}

Natural Anti-Gal and Salmonella-Specific Antibodies in Bile and Plasma of Hens Differing in Diet Efficiency. / Cotter, P.F.; van Eerden, E.

In: Poultry Science, Vol. 85, No. 3, 2006, p. 435-440.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Natural Anti-Gal and Salmonella-Specific Antibodies in Bile and Plasma of Hens Differing in Diet Efficiency

AU - Cotter, P.F.

AU - van Eerden, E.

PY - 2006

Y1 - 2006

N2 - Specific anti-Salmonella enteritidis (SE) and natural anti-¿-gal epitope (Gal¿1-3Galß-1-4GlcNAc-R; anti-Gal) antibodies were measured in plasma sample pools and individual bile specimens obtained from hens differing in diet efficiency. More SE somatic (O) and flagellar (H) antibodies were found in plasma pools from efficient hens (R¿) compared with nonefficient hens (R+) after oral challenge with live SE. Mean titers of somatic agglutinins in bile were 2.3 in R¿ hens and 1.9 in R+ hens (P = 0.06) following live challenge. Salmonella enteritidis antibodies were also found in bile of nonchallenged hens of both types but their levels were not significantly different. Flagellar (H) agglutinin scores were higher in SE-challenged hens compared with nonchallenged hens (3.1 vs. 2.1; P is less than or equal to 0.004) but efficiency types did not differ. Bile also contained high titers of the anti-Gal antibody indicated by the agglutination of glutaraldehyde-stabilized rabbit erythrocytes. The average titer of all SE-nonexposed hens was 9.0 corresponding to 1:5,120 when corrected for the initial dilution and expressed in conventional terms. Salmonella enteritidis exposure was associated with higher anti-Gal titers. The average anti-Gal titer for all SE-exposed hens was 10.0, corresponding to 1:10,240 in conventional terms; this difference was significant (P is less than or equal to 0.016). Diet efficiency type-associated differences in anti-Gal titers were not significant. Collectively, our data indicate that diet efficiency status is not associated with compromised Salmonella-specific immune responses. Rather it appears that the immune responses of diet efficient hens (R¿) are also more efficient. This is because R¿ hens produced higher levels of O- and H-type antibody only as the result of direct exposure to living SE. On the contrary, R+ hens produced H-type antibody because of challenge with heat-killed SE, a circumstance that will not result in disease. Moreover, the hen type difference does not seem to occur at the expense of innate immunity as measured by anti-Gal antibody levels

AB - Specific anti-Salmonella enteritidis (SE) and natural anti-¿-gal epitope (Gal¿1-3Galß-1-4GlcNAc-R; anti-Gal) antibodies were measured in plasma sample pools and individual bile specimens obtained from hens differing in diet efficiency. More SE somatic (O) and flagellar (H) antibodies were found in plasma pools from efficient hens (R¿) compared with nonefficient hens (R+) after oral challenge with live SE. Mean titers of somatic agglutinins in bile were 2.3 in R¿ hens and 1.9 in R+ hens (P = 0.06) following live challenge. Salmonella enteritidis antibodies were also found in bile of nonchallenged hens of both types but their levels were not significantly different. Flagellar (H) agglutinin scores were higher in SE-challenged hens compared with nonchallenged hens (3.1 vs. 2.1; P is less than or equal to 0.004) but efficiency types did not differ. Bile also contained high titers of the anti-Gal antibody indicated by the agglutination of glutaraldehyde-stabilized rabbit erythrocytes. The average titer of all SE-nonexposed hens was 9.0 corresponding to 1:5,120 when corrected for the initial dilution and expressed in conventional terms. Salmonella enteritidis exposure was associated with higher anti-Gal titers. The average anti-Gal titer for all SE-exposed hens was 10.0, corresponding to 1:10,240 in conventional terms; this difference was significant (P is less than or equal to 0.016). Diet efficiency type-associated differences in anti-Gal titers were not significant. Collectively, our data indicate that diet efficiency status is not associated with compromised Salmonella-specific immune responses. Rather it appears that the immune responses of diet efficient hens (R¿) are also more efficient. This is because R¿ hens produced higher levels of O- and H-type antibody only as the result of direct exposure to living SE. On the contrary, R+ hens produced H-type antibody because of challenge with heat-killed SE, a circumstance that will not result in disease. Moreover, the hen type difference does not seem to occur at the expense of innate immunity as measured by anti-Gal antibody levels

KW - residual feed-intake

KW - growing layer hens

KW - enteritidis infection

KW - immune-responses

KW - selection

KW - chickens

U2 - 10.1093/ps/85.3.435

DO - 10.1093/ps/85.3.435

M3 - Article

VL - 85

SP - 435

EP - 440

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 3

ER -