NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana

F. Yazdanpanah, Veronica Maurino, G. Buijs, M. Karimi Jashni, L.A.J. Willems, L. Sergeeva, Loïc Rajjou, H.W.M. Hilhorst, L. Bentsink

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Aging decreases the quality of seeds and results in agricultural
and economic losses. The damage that occurs at the
biochemical level can alter the seed physiological status.
Although loss of viability has been investigated frequently,
little information exists on the molecular and biochemical
factors involved in seed deterioration and loss of viability.
Oxidative stress has been implicated as a major contributor
to seed deterioration, and several pathways are involved in
protection against this. In this study, we show that seeds of
Arabidopsis thaliana lacking a functional NADP-MALIC
ENZYME 1 (NADP-ME1) have reduced seed viability relative
to the wild type. Seeds of the NADP-ME1 loss-of-function
mutant display higher levels of protein carbonylation than
those of the wild type. NADP-ME1 catalyzes the oxidative
decarboxylation of malate to pyruvate with the simultaneous
production of CO2 and NADPH. Upon seed imbibition,
malate and amino acids accumulate in embryos of aged
seeds of the NADP-ME1 loss-of-function mutant compared
with those of the wild type. NADP-ME1 expression is
increased in imbibed aged as compared with non-aged
seeds. NADP-ME1 activity at testa rupture promotes
normal germination of aged seeds. In seedlings of aged
seeds, NADP-ME1 is specifically active in the root meristematic
zone. We propose that NADP-ME1 activity is required
for protecting seeds against oxidation during seed dry
storage.
Keywords: Ageing Arabidopsis thaliana Enzyme activity
Gene expression Meristematic zone Metabolite
profiling Oxidative stress Seed longevity.
LanguageEnglish
Pages318–328
JournalPlant and Cell Physiology
Volume60
Issue number2
Early online date2 Nov 2018
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

seed storage
Germination
NADP
Arabidopsis
NADP (coenzyme)
Seeds
Arabidopsis thaliana
germination
seeds
viability
malates
oxidative stress
deterioration
Oxidative Stress
Protein Carbonylation
imbibition
seed quality
physiological state
testa
embryo (plant)

Cite this

Yazdanpanah, F. ; Maurino, Veronica ; Buijs, G. ; Karimi Jashni, M. ; Willems, L.A.J. ; Sergeeva, L. ; Rajjou, Loïc ; Hilhorst, H.W.M. ; Bentsink, L. / NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana. In: Plant and Cell Physiology. 2019 ; Vol. 60, No. 2. pp. 318–328.
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abstract = "Aging decreases the quality of seeds and results in agriculturaland economic losses. The damage that occurs at thebiochemical level can alter the seed physiological status.Although loss of viability has been investigated frequently,little information exists on the molecular and biochemicalfactors involved in seed deterioration and loss of viability.Oxidative stress has been implicated as a major contributorto seed deterioration, and several pathways are involved inprotection against this. In this study, we show that seeds ofArabidopsis thaliana lacking a functional NADP-MALICENZYME 1 (NADP-ME1) have reduced seed viability relativeto the wild type. Seeds of the NADP-ME1 loss-of-functionmutant display higher levels of protein carbonylation thanthose of the wild type. NADP-ME1 catalyzes the oxidativedecarboxylation of malate to pyruvate with the simultaneousproduction of CO2 and NADPH. Upon seed imbibition,malate and amino acids accumulate in embryos of agedseeds of the NADP-ME1 loss-of-function mutant comparedwith those of the wild type. NADP-ME1 expression isincreased in imbibed aged as compared with non-agedseeds. NADP-ME1 activity at testa rupture promotesnormal germination of aged seeds. In seedlings of agedseeds, NADP-ME1 is specifically active in the root meristematiczone. We propose that NADP-ME1 activity is requiredfor protecting seeds against oxidation during seed drystorage.Keywords: Ageing Arabidopsis thaliana Enzyme activity Gene expression Meristematic zone Metaboliteprofiling Oxidative stress Seed longevity.",
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NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana. / Yazdanpanah, F.; Maurino, Veronica; Buijs, G.; Karimi Jashni, M.; Willems, L.A.J.; Sergeeva, L.; Rajjou, Loïc; Hilhorst, H.W.M.; Bentsink, L.

In: Plant and Cell Physiology, Vol. 60, No. 2, 01.02.2019, p. 318–328.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana

AU - Yazdanpanah, F.

AU - Maurino, Veronica

AU - Buijs, G.

AU - Karimi Jashni, M.

AU - Willems, L.A.J.

AU - Sergeeva, L.

AU - Rajjou, Loïc

AU - Hilhorst, H.W.M.

AU - Bentsink, L.

PY - 2019/2/1

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N2 - Aging decreases the quality of seeds and results in agriculturaland economic losses. The damage that occurs at thebiochemical level can alter the seed physiological status.Although loss of viability has been investigated frequently,little information exists on the molecular and biochemicalfactors involved in seed deterioration and loss of viability.Oxidative stress has been implicated as a major contributorto seed deterioration, and several pathways are involved inprotection against this. In this study, we show that seeds ofArabidopsis thaliana lacking a functional NADP-MALICENZYME 1 (NADP-ME1) have reduced seed viability relativeto the wild type. Seeds of the NADP-ME1 loss-of-functionmutant display higher levels of protein carbonylation thanthose of the wild type. NADP-ME1 catalyzes the oxidativedecarboxylation of malate to pyruvate with the simultaneousproduction of CO2 and NADPH. Upon seed imbibition,malate and amino acids accumulate in embryos of agedseeds of the NADP-ME1 loss-of-function mutant comparedwith those of the wild type. NADP-ME1 expression isincreased in imbibed aged as compared with non-agedseeds. NADP-ME1 activity at testa rupture promotesnormal germination of aged seeds. In seedlings of agedseeds, NADP-ME1 is specifically active in the root meristematiczone. We propose that NADP-ME1 activity is requiredfor protecting seeds against oxidation during seed drystorage.Keywords: Ageing Arabidopsis thaliana Enzyme activity Gene expression Meristematic zone Metaboliteprofiling Oxidative stress Seed longevity.

AB - Aging decreases the quality of seeds and results in agriculturaland economic losses. The damage that occurs at thebiochemical level can alter the seed physiological status.Although loss of viability has been investigated frequently,little information exists on the molecular and biochemicalfactors involved in seed deterioration and loss of viability.Oxidative stress has been implicated as a major contributorto seed deterioration, and several pathways are involved inprotection against this. In this study, we show that seeds ofArabidopsis thaliana lacking a functional NADP-MALICENZYME 1 (NADP-ME1) have reduced seed viability relativeto the wild type. Seeds of the NADP-ME1 loss-of-functionmutant display higher levels of protein carbonylation thanthose of the wild type. NADP-ME1 catalyzes the oxidativedecarboxylation of malate to pyruvate with the simultaneousproduction of CO2 and NADPH. Upon seed imbibition,malate and amino acids accumulate in embryos of agedseeds of the NADP-ME1 loss-of-function mutant comparedwith those of the wild type. NADP-ME1 expression isincreased in imbibed aged as compared with non-agedseeds. NADP-ME1 activity at testa rupture promotesnormal germination of aged seeds. In seedlings of agedseeds, NADP-ME1 is specifically active in the root meristematiczone. We propose that NADP-ME1 activity is requiredfor protecting seeds against oxidation during seed drystorage.Keywords: Ageing Arabidopsis thaliana Enzyme activity Gene expression Meristematic zone Metaboliteprofiling Oxidative stress Seed longevity.

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DO - 10.1093/pcp/pcy213

M3 - Article

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SP - 318

EP - 328

JO - Plant and Cell Physiology

T2 - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

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