Mutation in sorghum LOW GERMINATION STIMULANT 1 alters strigolactones and causes Striga resistance

Daniel Gobena, Mahdere Shimels, Patrick J. Rich, Carolien Ruyter-Spira, Harro Bouwmeester, Satish Kanuganti, Tesfaye Mengiste, Gebisa Ejeta*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

51 Citations (Scopus)

Abstract

Striga is a major biotic constraint to sorghum production in semiarid tropical Africa and Asia. Genetic resistance to this parasitic weed is the most economically feasible control measure. Mutant alleles at the LGS1 (LOW GERMINATION STIMULANT 1) locus drastically reduce Striga germination stimulant activity. We provide evidence that the responsible gene at LGS1 codes for an enzyme annotated as a sulfotransferase and show that functional loss of this gene results in a change of the dominant strigolactone (SL) in root exudates from 5-deoxystrigol, a highly active Striga germination stimulant, to orobanchol, an SL with opposite stereochemistry. Orobanchol, although not previously reported in sorghum, functions in the multiple SL roles required for normal growth and environmental responsiveness but does not stimulate germination of Striga. This work describes the identification of a gene regulating Striga resistance and the underlying protective chemistry resulting from mutation.

Original languageEnglish
Pages (from-to)4471-4476
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number17
DOIs
Publication statusPublished - 2017

Keywords

  • Gene
  • Sorghum
  • Stereochemistry
  • Striga
  • Strigolactone

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