Mutan produced in potato amyloplasts adheres to starch granules

G.A. Kok-Jacon, J.P. Vincken, L.C.J.M. Suurs, R.G.F. Visser

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Production of water-insoluble mutan polymers in Kardal potato tubers was investigated after expression of a full-length (GtfI) and a truncated mutansucrase gene referred to as GtfICAT (GtfI without glucan-binding domain) from Streptococcus downei. Subsequent effects on starch biosynthesis at the molecular and biochemical levels were studied. Expression of the GtfICAT gene resulted in the adhesion of mutan material on starch granules, which stained red with erythrosine, and which was hydrolysed by exo-mutanase. In addition, GtfICAT-expressing plants exhibited a severely altered tuber phenotype and starch granule morphology in comparison to those expressing the full-length GtfI gene. In spite of that, no structural changes at the starch level were observed. Expression levels of the sucrose-regulated, AGPase and GBSSI genes were down-regulated in only the GTFICAT transformants, showing that GtfICAT expression interfered with the starch biosynthetic pathway. In accordance with the down-regulated AGPase gene, a lower starch content was observed in the GTFICAT transformants. Finally, the rheological properties of the GTFICAT starches were modified; they showed a higher retrogradation during cooling of the starch paste
Original languageEnglish
Pages (from-to)341-351
JournalPlant Biotechnology Journal
Volume3
Issue number3
DOIs
Publication statusPublished - 2005

Keywords

  • solanum-tuberosum-l
  • streptococcus-mutans
  • sucrose
  • gene
  • expression
  • plants
  • synthase
  • glucosyltransferase
  • sequence
  • cultures

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