Muscle growth mechanisms in response to isoenergetic changes in dietary non-protein energy source at low and high protein levels in juvenile rainbow trout

Hélène Alami-Durante*, Marianne Cluzeaud, Didier Bazin, Johan W. Schrama, Subramanian Saravanan, Inge Geurden

*Corresponding author for this work

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3 Citations (Scopus)

Abstract

This study investigates muscle growth mechanisms in juvenile rainbow trout in response to isoenergetic changes in dietary non-protein energy (NPE) source (F, fat vs. C, carbohydrates) at two levels of digestible protein to digestible energy (DP/DE) ratio. Fish (initial weight 32.4 g) were fed four diets having similar DE levels (~18 kJ g−1) with a high (HP/E~26 mg kJ−1) vs. low (LP/E~14 mg kJ−1) DP/DE ratio using F or C as major NPE source (7 week-experiment). The lowering of dietary DP/DE ratio increased myoblast determination protein 1a (myod1a) and decreased myostatin 1b (mstn1b) and cathepsin D (ctsd) muscle mRNA levels. The isoenergetic change in dietary NPE from F to C decreased myod1a and proliferative cell nuclear antigen (pcna) muscle mRNA levels. An interaction between DP/DE ratio and NPE source was observed in muscle transcript levels of myogenic factor 6 (mrf4/myf6), fast myosin heavy chain (fmhc) and fast myosin light chain 2 (fmlc2). White muscle total cross-sectional area decreased at low dietary DP/DE ratio and also when NPE source changed from F to C, linked i) to a decreased total number of white muscle fibres, indicating that low dietary DP/DE restricted muscle hyperplasia and that dietary carbohydrate were less efficiently used than fat to sustain muscle hyperplasia, and ii) to decreased percentage of large muscle fibres, indicating limited fibre hypertrophy. Not only the DP level or the DP/DE ratio, but also the isoenergetic change in dietary NPE source (fat vs carbohydrates) thus appears as a potent regulator of muscle hyperplasia and hypertrophy.
Original languageEnglish
Pages (from-to)91-99
JournalComparative Biochemistry and Physiology. A, Molecular and Integrative Physiology
Volume230
DOIs
Publication statusPublished - Apr 2019

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Dietary Proteins
Oncorhynchus mykiss
Muscle
Muscles
Growth
Proteins
Hyperplasia
Myoblasts
Fats
Hypertrophy
Fibers
Carbohydrates
Myostatin
Fast-Twitch Muscle Fibers
Dietary Carbohydrates
Nuclear Antigens
Cathepsin D
Messenger RNA
Myosin Heavy Chains
Nutrition

Cite this

@article{911913406f00491a9617c75ea6d8f4c3,
title = "Muscle growth mechanisms in response to isoenergetic changes in dietary non-protein energy source at low and high protein levels in juvenile rainbow trout",
abstract = "This study investigates muscle growth mechanisms in juvenile rainbow trout in response to isoenergetic changes in dietary non-protein energy (NPE) source (F, fat vs. C, carbohydrates) at two levels of digestible protein to digestible energy (DP/DE) ratio. Fish (initial weight 32.4 g) were fed four diets having similar DE levels (~18 kJ g−1) with a high (HP/E~26 mg kJ−1) vs. low (LP/E~14 mg kJ−1) DP/DE ratio using F or C as major NPE source (7 week-experiment). The lowering of dietary DP/DE ratio increased myoblast determination protein 1a (myod1a) and decreased myostatin 1b (mstn1b) and cathepsin D (ctsd) muscle mRNA levels. The isoenergetic change in dietary NPE from F to C decreased myod1a and proliferative cell nuclear antigen (pcna) muscle mRNA levels. An interaction between DP/DE ratio and NPE source was observed in muscle transcript levels of myogenic factor 6 (mrf4/myf6), fast myosin heavy chain (fmhc) and fast myosin light chain 2 (fmlc2). White muscle total cross-sectional area decreased at low dietary DP/DE ratio and also when NPE source changed from F to C, linked i) to a decreased total number of white muscle fibres, indicating that low dietary DP/DE restricted muscle hyperplasia and that dietary carbohydrate were less efficiently used than fat to sustain muscle hyperplasia, and ii) to decreased percentage of large muscle fibres, indicating limited fibre hypertrophy. Not only the DP level or the DP/DE ratio, but also the isoenergetic change in dietary NPE source (fat vs carbohydrates) thus appears as a potent regulator of muscle hyperplasia and hypertrophy.",
author = "H{\'e}l{\`e}ne Alami-Durante and Marianne Cluzeaud and Didier Bazin and Schrama, {Johan W.} and Subramanian Saravanan and Inge Geurden",
year = "2019",
month = "4",
doi = "10.1016/j.cbpa.2019.01.009",
language = "English",
volume = "230",
pages = "91--99",
journal = "Comparative Biochemistry and Physiology. A, Molecular and Integrative Physiology",
issn = "1095-6433",
publisher = "Elsevier",

}

Muscle growth mechanisms in response to isoenergetic changes in dietary non-protein energy source at low and high protein levels in juvenile rainbow trout. / Alami-Durante, Hélène; Cluzeaud, Marianne; Bazin, Didier; Schrama, Johan W.; Saravanan, Subramanian; Geurden, Inge.

In: Comparative Biochemistry and Physiology. A, Molecular and Integrative Physiology, Vol. 230, 04.2019, p. 91-99.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Muscle growth mechanisms in response to isoenergetic changes in dietary non-protein energy source at low and high protein levels in juvenile rainbow trout

AU - Alami-Durante, Hélène

AU - Cluzeaud, Marianne

AU - Bazin, Didier

AU - Schrama, Johan W.

AU - Saravanan, Subramanian

AU - Geurden, Inge

PY - 2019/4

Y1 - 2019/4

N2 - This study investigates muscle growth mechanisms in juvenile rainbow trout in response to isoenergetic changes in dietary non-protein energy (NPE) source (F, fat vs. C, carbohydrates) at two levels of digestible protein to digestible energy (DP/DE) ratio. Fish (initial weight 32.4 g) were fed four diets having similar DE levels (~18 kJ g−1) with a high (HP/E~26 mg kJ−1) vs. low (LP/E~14 mg kJ−1) DP/DE ratio using F or C as major NPE source (7 week-experiment). The lowering of dietary DP/DE ratio increased myoblast determination protein 1a (myod1a) and decreased myostatin 1b (mstn1b) and cathepsin D (ctsd) muscle mRNA levels. The isoenergetic change in dietary NPE from F to C decreased myod1a and proliferative cell nuclear antigen (pcna) muscle mRNA levels. An interaction between DP/DE ratio and NPE source was observed in muscle transcript levels of myogenic factor 6 (mrf4/myf6), fast myosin heavy chain (fmhc) and fast myosin light chain 2 (fmlc2). White muscle total cross-sectional area decreased at low dietary DP/DE ratio and also when NPE source changed from F to C, linked i) to a decreased total number of white muscle fibres, indicating that low dietary DP/DE restricted muscle hyperplasia and that dietary carbohydrate were less efficiently used than fat to sustain muscle hyperplasia, and ii) to decreased percentage of large muscle fibres, indicating limited fibre hypertrophy. Not only the DP level or the DP/DE ratio, but also the isoenergetic change in dietary NPE source (fat vs carbohydrates) thus appears as a potent regulator of muscle hyperplasia and hypertrophy.

AB - This study investigates muscle growth mechanisms in juvenile rainbow trout in response to isoenergetic changes in dietary non-protein energy (NPE) source (F, fat vs. C, carbohydrates) at two levels of digestible protein to digestible energy (DP/DE) ratio. Fish (initial weight 32.4 g) were fed four diets having similar DE levels (~18 kJ g−1) with a high (HP/E~26 mg kJ−1) vs. low (LP/E~14 mg kJ−1) DP/DE ratio using F or C as major NPE source (7 week-experiment). The lowering of dietary DP/DE ratio increased myoblast determination protein 1a (myod1a) and decreased myostatin 1b (mstn1b) and cathepsin D (ctsd) muscle mRNA levels. The isoenergetic change in dietary NPE from F to C decreased myod1a and proliferative cell nuclear antigen (pcna) muscle mRNA levels. An interaction between DP/DE ratio and NPE source was observed in muscle transcript levels of myogenic factor 6 (mrf4/myf6), fast myosin heavy chain (fmhc) and fast myosin light chain 2 (fmlc2). White muscle total cross-sectional area decreased at low dietary DP/DE ratio and also when NPE source changed from F to C, linked i) to a decreased total number of white muscle fibres, indicating that low dietary DP/DE restricted muscle hyperplasia and that dietary carbohydrate were less efficiently used than fat to sustain muscle hyperplasia, and ii) to decreased percentage of large muscle fibres, indicating limited fibre hypertrophy. Not only the DP level or the DP/DE ratio, but also the isoenergetic change in dietary NPE source (fat vs carbohydrates) thus appears as a potent regulator of muscle hyperplasia and hypertrophy.

U2 - 10.1016/j.cbpa.2019.01.009

DO - 10.1016/j.cbpa.2019.01.009

M3 - Article

VL - 230

SP - 91

EP - 99

JO - Comparative Biochemistry and Physiology. A, Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology. A, Molecular and Integrative Physiology

SN - 1095-6433

ER -