Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems

Catalina A. Suarez Ruiz; Jennifer Kwaijtaal; Oriol Cabau Peinado; Corjan Van Den Berg; Rene H. Wijffels; Michel H.M. Eppink

doi:10.1021/acssuschemeng.9b06379

Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems

Catalina A. Suarez Ruiz, Jennifer Kwaijtaal, Oriol Cabau Peinado, Corjan Van Den Berg, Rene H. Wijffels, Michel H.M. Eppink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

32 Citations (Scopus)

Abstract

We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.

Original language	English
Pages (from-to)	2441-2452
Journal	ACS Sustainable Chemistry and Engineering
Volume	8
Issue number	6
DOIs	https://doi.org/10.1021/acssuschemeng.9b06379
Publication status	Published - 17 Feb 2020

Keywords

Aqueous two-phase systems
Biomolecules
Cholinium-based ionic liquids
Downstream processes
Microalgae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acssuschemeng.9b06379Licence: CC BY-NC-ND

https://edepot.wur.nl/517452Licence: CC BY-NC-ND

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title = "Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems",

abstract = "We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.",

keywords = "Aqueous two-phase systems, Biomolecules, Cholinium-based ionic liquids, Downstream processes, Microalgae",

author = "{Suarez Ruiz}, {Catalina A.} and Jennifer Kwaijtaal and Peinado, {Oriol Cabau} and {Van Den Berg}, Corjan and Wijffels, {Rene H.} and Eppink, {Michel H.M.}",

year = "2020",

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doi = "10.1021/acssuschemeng.9b06379",

language = "English",

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journal = "ACS sustainable chemistry & engineering",

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T1 - Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems

AU - Suarez Ruiz, Catalina A.

AU - Kwaijtaal, Jennifer

AU - Peinado, Oriol Cabau

AU - Van Den Berg, Corjan

AU - Wijffels, Rene H.

AU - Eppink, Michel H.M.

PY - 2020/2/17

Y1 - 2020/2/17

N2 - We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.

AB - We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.

KW - Aqueous two-phase systems

KW - Biomolecules

KW - Cholinium-based ionic liquids

KW - Downstream processes

KW - Microalgae

U2 - 10.1021/acssuschemeng.9b06379

DO - 10.1021/acssuschemeng.9b06379

M3 - Article

AN - SCOPUS:85079613723

SN - 2168-0485

VL - 8

SP - 2441

EP - 2452

JO - ACS sustainable chemistry & engineering

JF - ACS sustainable chemistry & engineering

IS - 6

ER -

Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems

Abstract

Keywords

UN SDGs

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