TY - JOUR
T1 - Multistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems
AU - Suarez Ruiz, Catalina A.
AU - Kwaijtaal, Jennifer
AU - Peinado, Oriol Cabau
AU - Van Den Berg, Corjan
AU - Wijffels, Rene H.
AU - Eppink, Michel H.M.
PY - 2020/2/17
Y1 - 2020/2/17
N2 - We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.
AB - We aim to develop liquid-liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.
KW - Aqueous two-phase systems
KW - Biomolecules
KW - Cholinium-based ionic liquids
KW - Downstream processes
KW - Microalgae
U2 - 10.1021/acssuschemeng.9b06379
DO - 10.1021/acssuschemeng.9b06379
M3 - Article
AN - SCOPUS:85079613723
SN - 2168-0485
VL - 8
SP - 2441
EP - 2452
JO - ACS Sustainable Chemistry and Engineering
JF - ACS Sustainable Chemistry and Engineering
IS - 6
ER -