Multisite gateway-compatible cell type-specific gene-inducible system for plants

Riccardo Siligato, Xin Wang, Shri Ram Yadav, Satu Lehesranta, Guojie Ma, Robertas Ursache, Iris Sevilem, Jing Zhang, Maartje Gorte, Kalika Prasad, Michael Wrzaczek, Renze Heidstra, Angus Murphy, Ben Scheres, Ari Pekka Mähönen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

A powerful method to study gene function is expression or overexpression in an inducible, cell type-specific system followed by observation of consequent phenotypic changes and visualization of linked reporters in the target tissue. Multiple inducible gene overexpression systems have been developed for plants, but very few of these combine plant selection markers, control of expression domains, access to multiple promoters and protein fusion reporters, chemical induction, and high-throughput cloning capabilities. Here, we introduce a MultiSite Gateway-compatible inducible system for Arabidopsis (Arabidopsis thaliana) plants that provides the capability to generate such constructs in a single cloning step. The system is based on the tightly controlled, estrogen-inducible XVE system. We demonstrate that the transformants generated with this system exhibit the expected cell type-specific expression, similar to what is observed with constitutively expressed native promoters. With this new system, cloning of inducible constructs is no longer limited to a few special cases but can be used as a standard approach when gene function is studied. In addition, we present a set of entry clones consisting of histochemical and fluorescent reporter variants designed for gene and promoter expression studies.

Original languageEnglish
Pages (from-to)627-641
JournalPlant Physiology
Volume170
Issue number2
DOIs
Publication statusPublished - 2016

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