Multiplex genome editing of microorganisms using CRISPR-Cas

Belén Adiego-Pérez, Paola Randazzo, Jean Marc Daran, René Verwaal, Johannes A. Roubos, Pascale Daran-Lapujade, John van der Oost*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing.

Original languageEnglish
JournalFEMS Microbiology Letters
Volume366
Issue number8
DOIs
Publication statusPublished - 14 May 2019

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Clustered Regularly Interspaced Short Palindromic Repeats
Gene Expression Regulation
Bacteria
Gene Editing

Keywords

  • Cas12a
  • Cas9
  • cell factories
  • CRISPR-Cas
  • genome editing
  • multiplex

Cite this

Adiego-Pérez, Belén ; Randazzo, Paola ; Daran, Jean Marc ; Verwaal, René ; Roubos, Johannes A. ; Daran-Lapujade, Pascale ; van der Oost, John. / Multiplex genome editing of microorganisms using CRISPR-Cas. In: FEMS Microbiology Letters. 2019 ; Vol. 366, No. 8.
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Multiplex genome editing of microorganisms using CRISPR-Cas. / Adiego-Pérez, Belén; Randazzo, Paola; Daran, Jean Marc; Verwaal, René; Roubos, Johannes A.; Daran-Lapujade, Pascale; van der Oost, John.

In: FEMS Microbiology Letters, Vol. 366, No. 8, 14.05.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Adiego-Pérez, Belén

AU - Randazzo, Paola

AU - Daran, Jean Marc

AU - Verwaal, René

AU - Roubos, Johannes A.

AU - Daran-Lapujade, Pascale

AU - van der Oost, John

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AB - Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing.

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Adiego-Pérez B, Randazzo P, Daran JM, Verwaal R, Roubos JA, Daran-Lapujade P et al. Multiplex genome editing of microorganisms using CRISPR-Cas. FEMS Microbiology Letters. 2019 May 14;366(8). https://doi.org/10.1093/femsle/fnz086