Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M. Degennaro, Nerges Winblad, Sourav R. Choudhury, Omar O. Abudayyeh, Wen Y. Wu, John van der Oost

Research output: Contribution to journalArticleAcademicpeer-review

287 Citations (Scopus)

Abstract

Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

Original languageEnglish
Pages (from-to)31-34
JournalNature Biotechnology
Volume35
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint Dive into the research topics of 'Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array'. Together they form a unique fingerprint.

  • Cite this

    Zetsche, B., Heidenreich, M., Mohanraju, P., Fedorova, I., Kneppers, J., Degennaro, E. M., Winblad, N., Choudhury, S. R., Abudayyeh, O. O., Wu, W. Y., & van der Oost, J. (2017). Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nature Biotechnology, 35(1), 31-34. https://doi.org/10.1038/nbt.3737