Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M. Degennaro, Nerges Winblad, Sourav R. Choudhury, Omar O. Abudayyeh, Wen Y. Wu, John van der Oost

Research output: Contribution to journalArticleAcademicpeer-review

210 Citations (Scopus)

Abstract

Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

LanguageEnglish
Pages31-34
JournalNature Biotechnology
Volume35
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
RNA
Genes
Brain
Cells
Gene Editing

Cite this

Zetsche, Bernd ; Heidenreich, Matthias ; Mohanraju, Prarthana ; Fedorova, Iana ; Kneppers, Jeroen ; Degennaro, Ellen M. ; Winblad, Nerges ; Choudhury, Sourav R. ; Abudayyeh, Omar O. ; Wu, Wen Y. ; van der Oost, John. / Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. In: Nature Biotechnology. 2017 ; Vol. 35, No. 1. pp. 31-34.
@article{3d880becef604fbe9897e87d138bd4ec,
title = "Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array",
abstract = "Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.",
author = "Bernd Zetsche and Matthias Heidenreich and Prarthana Mohanraju and Iana Fedorova and Jeroen Kneppers and Degennaro, {Ellen M.} and Nerges Winblad and Choudhury, {Sourav R.} and Abudayyeh, {Omar O.} and Wu, {Wen Y.} and {van der Oost}, John",
year = "2017",
doi = "10.1038/nbt.3737",
language = "English",
volume = "35",
pages = "31--34",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "1",

}

Zetsche, B, Heidenreich, M, Mohanraju, P, Fedorova, I, Kneppers, J, Degennaro, EM, Winblad, N, Choudhury, SR, Abudayyeh, OO, Wu, WY & van der Oost, J 2017, 'Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array', Nature Biotechnology, vol. 35, no. 1, pp. 31-34. https://doi.org/10.1038/nbt.3737

Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. / Zetsche, Bernd; Heidenreich, Matthias; Mohanraju, Prarthana; Fedorova, Iana; Kneppers, Jeroen; Degennaro, Ellen M.; Winblad, Nerges; Choudhury, Sourav R.; Abudayyeh, Omar O.; Wu, Wen Y.; van der Oost, John.

In: Nature Biotechnology, Vol. 35, No. 1, 2017, p. 31-34.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

AU - Zetsche, Bernd

AU - Heidenreich, Matthias

AU - Mohanraju, Prarthana

AU - Fedorova, Iana

AU - Kneppers, Jeroen

AU - Degennaro, Ellen M.

AU - Winblad, Nerges

AU - Choudhury, Sourav R.

AU - Abudayyeh, Omar O.

AU - Wu, Wen Y.

AU - van der Oost, John

PY - 2017

Y1 - 2017

N2 - Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

AB - Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

U2 - 10.1038/nbt.3737

DO - 10.1038/nbt.3737

M3 - Article

VL - 35

SP - 31

EP - 34

JO - Nature Biotechnology

T2 - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 1

ER -

Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, Degennaro EM et al. Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nature Biotechnology. 2017;35(1):31-34. https://doi.org/10.1038/nbt.3737