Multiplex flow cytometric immunoassay for serum biomarker profiling of recombinant bovine somatotropin

N.G.E. Smits, S.K.J. Ludwig, G. van der Veer, M.G.E.G. Bremer, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Recombinant bovine somatotropin (rbST) is licensed for enhancing milk production in dairy cows in some countries, for instance the United States, but is banned in Europe. Serum biomarker profiling can be an adequate approach to discriminate between treated and untreated groups. In this study a multiplex screening tool of a small set of biomarkers for pinpointing recombinant bovine somatotropin (rbST) (ab)use was developed and evaluated: insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP2) and rbST-induced antibodies were selected as rbST dependent markers and combined in one parallel assay format. For this, the color-encoded microspheres were used in a suspension array, with a dedicated flow cytometer. Serum samples obtained from an animal experiment with rbST-treated and untreated dairy cows were measured with the developed triplex immunoassay and biomarker responses on rbST treatment were evaluated. This resulted in characteristic treatment-dependent responses for all three individual biomarkers. Combining these results with the statistical prediction model k-nearest neighbours (kNN), resulted in good discrimination of treated and untreated animals: an overall sensitivity (true positive rate) of 89.1% and an overall specificity (true negative rate) of 97.7% were reached. Therefore, this is the first multiplex method which can be applied with high confidence for screening of unknown herds of cattle pinpointing at rbST (ab)use.
Original languageEnglish
Pages (from-to)111-117
JournalThe Analyst
Volume138
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

immunoassay
Biomarkers
Immunoassay
Growth Hormone
biomarker
serum
Dairies
Serum
Screening
Animals
Insulin
Microspheres
Antibodies
Assays
Insulin-Like Growth Factor Binding Protein 2
milk production
Color
Statistical Models
Somatomedins
bovine growth hormone

Keywords

  • growth-factor-i
  • enzyme-immunoassay
  • antibody-formation
  • biological-fluids
  • binding-proteins
  • lactating cows
  • hormone
  • cattle
  • identification
  • osteocalcin

Cite this

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title = "Multiplex flow cytometric immunoassay for serum biomarker profiling of recombinant bovine somatotropin",
abstract = "Recombinant bovine somatotropin (rbST) is licensed for enhancing milk production in dairy cows in some countries, for instance the United States, but is banned in Europe. Serum biomarker profiling can be an adequate approach to discriminate between treated and untreated groups. In this study a multiplex screening tool of a small set of biomarkers for pinpointing recombinant bovine somatotropin (rbST) (ab)use was developed and evaluated: insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP2) and rbST-induced antibodies were selected as rbST dependent markers and combined in one parallel assay format. For this, the color-encoded microspheres were used in a suspension array, with a dedicated flow cytometer. Serum samples obtained from an animal experiment with rbST-treated and untreated dairy cows were measured with the developed triplex immunoassay and biomarker responses on rbST treatment were evaluated. This resulted in characteristic treatment-dependent responses for all three individual biomarkers. Combining these results with the statistical prediction model k-nearest neighbours (kNN), resulted in good discrimination of treated and untreated animals: an overall sensitivity (true positive rate) of 89.1{\%} and an overall specificity (true negative rate) of 97.7{\%} were reached. Therefore, this is the first multiplex method which can be applied with high confidence for screening of unknown herds of cattle pinpointing at rbST (ab)use.",
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author = "N.G.E. Smits and S.K.J. Ludwig and {van der Veer}, G. and M.G.E.G. Bremer and M.W.F. Nielen",
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Multiplex flow cytometric immunoassay for serum biomarker profiling of recombinant bovine somatotropin. / Smits, N.G.E.; Ludwig, S.K.J.; van der Veer, G.; Bremer, M.G.E.G.; Nielen, M.W.F.

In: The Analyst, Vol. 138, No. 1, 2013, p. 111-117.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Multiplex flow cytometric immunoassay for serum biomarker profiling of recombinant bovine somatotropin

AU - Smits, N.G.E.

AU - Ludwig, S.K.J.

AU - van der Veer, G.

AU - Bremer, M.G.E.G.

AU - Nielen, M.W.F.

PY - 2013

Y1 - 2013

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KW - binding-proteins

KW - lactating cows

KW - hormone

KW - cattle

KW - identification

KW - osteocalcin

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