Abstract
| Original language | English |
|---|---|
| Title of host publication | Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition |
| Editors | C. Lacomme |
| Place of Publication | New York 2015 |
| Pages | 283-300 |
| Number of pages | 352 |
| DOIs | |
| Publication status | Published - 2015 |
Publication series
| Name | Methods in Molecular Biology;Springer Protocols |
|---|---|
| Publisher | Humana Press |
| Number | 1302 |
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Multiplex detection of plant pathogens through the luminex magplex bead system. / van der Vlugt, R.A.A.; van Raaij, H.M.G.; de Weerdt, M.; Bergervoet, J.H.W.
Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. ed. / C. Lacomme. New York 2015, 2015. p. 283-300 (Methods in Molecular Biology;Springer Protocols; No. 1302).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
TY - CHAP
T1 - Multiplex detection of plant pathogens through the luminex magplex bead system
AU - van der Vlugt, R.A.A.
AU - van Raaij, H.M.G.
AU - de Weerdt, M.
AU - Bergervoet, J.H.W.
PY - 2015
Y1 - 2015
N2 - Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs
AB - Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs
U2 - 10.1007/978-1-4939-2620-6_21
DO - 10.1007/978-1-4939-2620-6_21
M3 - Chapter
SN - 9781493926190
T3 - Methods in Molecular Biology;Springer Protocols
SP - 283
EP - 300
BT - Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition
A2 - Lacomme, C.
CY - New York 2015
ER -