Multiplex detection of plant pathogens through the luminex magplex bead system

R.A.A. van der Vlugt; H.M.G. van Raaij; M. de Weerdt; J.H.W. Bergervoet

doi:10.1007/978-1-4939-2620-6_21

Multiplex detection of plant pathogens through the luminex magplex bead system

R.A.A. van der Vlugt, H.M.G. van Raaij, M. de Weerdt, J.H.W. Bergervoet

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

6 Citations (Scopus)

Abstract

Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs

Original language	English
Title of host publication	Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition
Editors	C. Lacomme
Place of Publication	New York 2015
Pages	283-300
Number of pages	352
DOIs	https://doi.org/10.1007/978-1-4939-2620-6_21
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology;Springer Protocols
Publisher	Humana Press
Number	1302

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10.1007/978-1-4939-2620-6_21

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van der Vlugt, R. A. A., van Raaij, H. M. G., de Weerdt, M., & Bergervoet, J. H. W. (2015). Multiplex detection of plant pathogens through the luminex magplex bead system. In C. Lacomme (Ed.), Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition (pp. 283-300). (Methods in Molecular Biology;Springer Protocols; No. 1302).. https://doi.org/10.1007/978-1-4939-2620-6_21

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abstract = "Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs",

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van der Vlugt, RAA , van Raaij, HMG, de Weerdt, M & Bergervoet, JHW 2015, Multiplex detection of plant pathogens through the luminex magplex bead system. in C Lacomme (ed.), Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. Methods in Molecular Biology;Springer Protocols, no. 1302, New York 2015, pp. 283-300. https://doi.org/10.1007/978-1-4939-2620-6_21

Multiplex detection of plant pathogens through the luminex magplex bead system. / van der Vlugt, R.A.A.; van Raaij, H.M.G.; de Weerdt, M.; Bergervoet, J.H.W.

Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. ed. / C. Lacomme. New York 2015, 2015. p. 283-300 (Methods in Molecular Biology;Springer Protocols; No. 1302).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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van der Vlugt RAA , van Raaij HMG, de Weerdt M, Bergervoet JHW. Multiplex detection of plant pathogens through the luminex magplex bead system. In Lacomme C, editor, Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. New York 2015. 2015. p. 283-300. (Methods in Molecular Biology;Springer Protocols; 1302). https://doi.org/10.1007/978-1-4939-2620-6_21