Multiplex detection of plant pathogens through the luminex magplex bead system

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

5 Citations (Scopus)

Abstract

Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs
Original languageEnglish
Title of host publicationPlant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition
EditorsC. Lacomme
Place of PublicationNew York 2015
Pages283-300
Number of pages352
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology;Springer Protocols
PublisherHumana Press
Number1302

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plant pathogens
oligonucleotides
labor
detection limit
viruses
antibodies
liquids
bacteria
assays
methodology
sampling

Cite this

van der Vlugt, R. A. A., van Raaij, H. M. G., de Weerdt, M., & Bergervoet, J. H. W. (2015). Multiplex detection of plant pathogens through the luminex magplex bead system. In C. Lacomme (Ed.), Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition (pp. 283-300). (Methods in Molecular Biology;Springer Protocols; No. 1302). New York 2015. https://doi.org/10.1007/978-1-4939-2620-6_21
van der Vlugt, R.A.A. ; van Raaij, H.M.G. ; de Weerdt, M. ; Bergervoet, J.H.W. / Multiplex detection of plant pathogens through the luminex magplex bead system. Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. editor / C. Lacomme. New York 2015, 2015. pp. 283-300 (Methods in Molecular Biology;Springer Protocols; 1302).
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van der Vlugt, RAA, van Raaij, HMG, de Weerdt, M & Bergervoet, JHW 2015, Multiplex detection of plant pathogens through the luminex magplex bead system. in C Lacomme (ed.), Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. Methods in Molecular Biology;Springer Protocols, no. 1302, New York 2015, pp. 283-300. https://doi.org/10.1007/978-1-4939-2620-6_21

Multiplex detection of plant pathogens through the luminex magplex bead system. / van der Vlugt, R.A.A.; van Raaij, H.M.G.; de Weerdt, M.; Bergervoet, J.H.W.

Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. ed. / C. Lacomme. New York 2015, 2015. p. 283-300 (Methods in Molecular Biology;Springer Protocols; No. 1302).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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AB - Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs

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van der Vlugt RAA, van Raaij HMG, de Weerdt M, Bergervoet JHW. Multiplex detection of plant pathogens through the luminex magplex bead system. In Lacomme C, editor, Plant Pathology: Techniques and Protocols, Methods in Molecular Biology; Second Edition. New York 2015. 2015. p. 283-300. (Methods in Molecular Biology;Springer Protocols; 1302). https://doi.org/10.1007/978-1-4939-2620-6_21