Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

Anna Yu. Alekseeva; Larisa V. Kameneva; Svetlana V. Kostyuk; Natalia N. Veiko

doi:10.1007/978-3-319-42044-8_25

Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

Anna Yu. Alekseeva, Larisa V. Kameneva, Svetlana V. Kostyuk, Natalia N. Veiko

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

8 Citations (Scopus)

Abstract

Oxidized cell-free DNA acts as a stress signal molecule and triggers an adaptive response in human cells. Various membrane DNA recognizing receptors are known as potential sensors for such DNA fragments. In order to clarify which of these sensors are able to interact with cfDNA fragments, circulating in human blood flow in heath and disease, we studied the influence of various cfDNA types on endothelial cells. We incubated these fragments at a physiologically optimal concentration with HUVEC cells for 3–24 h and detected the expression of either TLR9 or AIM2, RIG1 and STING receptors at mRNA and protein levels. We estimated that the activation of both TLR9 and other types of intracellular receptors initiates stress signaling in the endothelium independently. Signal transduction through these receptors activates NOX4 as the main source of ROS production in HUVECs.

Original language	English
Title of host publication	Circulating Nucleic Acids in Serum and Plasma – CNAPS IX
Editors	P.B. Gahan, M. Fleischhacker, B. Schmidt
Chapter	25
Pages	127-131
ISBN (Electronic)	9783319824871
DOIs	https://doi.org/10.1007/978-3-319-42044-8_25
Publication status	Published - 18 Oct 2016
Externally published	Yes

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	924
ISSN (Print)	0065-2598
ISSN (Electronic)	2214-8019

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SDG 3 Good Health and Well-being

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10.1007/978-3-319-42044-8_25

Cite this

Alekseeva, A. Y., Kameneva, L. V., Kostyuk, S. V., & Veiko, N. N. (2016). Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells. In P. B. Gahan, M. Fleischhacker, & B. Schmidt (Eds.), Circulating Nucleic Acids in Serum and Plasma – CNAPS IX (pp. 127-131). (Advances in Experimental Medicine and Biology; Vol. 924). https://doi.org/10.1007/978-3-319-42044-8_25

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abstract = "Oxidized cell-free DNA acts as a stress signal molecule and triggers an adaptive response in human cells. Various membrane DNA recognizing receptors are known as potential sensors for such DNA fragments. In order to clarify which of these sensors are able to interact with cfDNA fragments, circulating in human blood flow in heath and disease, we studied the influence of various cfDNA types on endothelial cells. We incubated these fragments at a physiologically optimal concentration with HUVEC cells for 3–24 h and detected the expression of either TLR9 or AIM2, RIG1 and STING receptors at mRNA and protein levels. We estimated that the activation of both TLR9 and other types of intracellular receptors initiates stress signaling in the endothelium independently. Signal transduction through these receptors activates NOX4 as the main source of ROS production in HUVECs.",

author = "Alekseeva, \{Anna Yu.\} and Kameneva, \{Larisa V.\} and Kostyuk, \{Svetlana V.\} and Veiko, \{Natalia N.\}",

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doi = "10.1007/978-3-319-42044-8\_25",

language = "English",

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Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells. / Alekseeva, Anna Yu.; Kameneva, Larisa V.; Kostyuk, Svetlana V. et al.
Circulating Nucleic Acids in Serum and Plasma – CNAPS IX . ed. / P.B. Gahan; M. Fleischhacker; B. Schmidt. 2016. p. 127-131 (Advances in Experimental Medicine and Biology; Vol. 924).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

TY - CHAP

T1 - Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

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AU - Kostyuk, Svetlana V.

AU - Veiko, Natalia N.

PY - 2016/10/18

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AB - Oxidized cell-free DNA acts as a stress signal molecule and triggers an adaptive response in human cells. Various membrane DNA recognizing receptors are known as potential sensors for such DNA fragments. In order to clarify which of these sensors are able to interact with cfDNA fragments, circulating in human blood flow in heath and disease, we studied the influence of various cfDNA types on endothelial cells. We incubated these fragments at a physiologically optimal concentration with HUVEC cells for 3–24 h and detected the expression of either TLR9 or AIM2, RIG1 and STING receptors at mRNA and protein levels. We estimated that the activation of both TLR9 and other types of intracellular receptors initiates stress signaling in the endothelium independently. Signal transduction through these receptors activates NOX4 as the main source of ROS production in HUVECs.

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M3 - Chapter

SN - 9783319420424

T3 - Advances in Experimental Medicine and Biology

SP - 127

EP - 131

BT - Circulating Nucleic Acids in Serum and Plasma – CNAPS IX

A2 - Gahan, P.B.

A2 - Fleischhacker, M.

A2 - Schmidt, B.

ER -

Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

Abstract

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