Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

R.M. Fratila, M.V. Gomez, S. Sykora, A.H. Velders

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications. Here we show that a non-resonant planar transceiver microcoil integrated in a microfluidic chip (detection volume 25¿nl) can detect different nuclides in the full broad-band range of Larmor frequencies (at 9.4¿T from 61 to 400¿MHz). Routine one-dimensional (1D) and two-dimensional (2D), homo- and heteronuclear experiments can be carried out using the broad-band coil set-up. Noteworthy, heteronuclear 2D experiments can be performed in a straightforward manner on virtually any combination of nuclides (from classical 1H–13C to more exotic combinations like 19F–31P) both in coupled and decoupled mode. Importantly, the concept of a non-resonant system provides magnetic field-independent NMR probes; moreover, the small-volume alleviates problems related to field inhomogeneity, making the broad-band coil an attractive option for, for example, portable and table-top NMR systems
Original languageEnglish
Article number3025
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014

Keywords

  • nuclear-magnetic-resonance
  • liquid-state nmr
  • to-noise ratio
  • multidimensional nmr
  • probe
  • f-19
  • design
  • chip
  • flow
  • h-1-nmr

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