Multimodal synchrotron X-ray fluorescence imaging reveals elemental distribution in seeds and seedlings of the Zn-Cd-Ni hyperaccumulator Noccaea caerulescens

Antony van der Ent*, Martin D. de Jonge, Guillaume Echevarria, Mark G.M. Aarts, Jolanta Mesjasz-Przybyłowicz, Wojciech J. Przybyłowicz, Dennis Brueckner, Hugh H. Harris

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The molecular biology and genetics of the Ni-Cd-Zn hyperaccumulator Noccaea caerulescens has been extensively studied, but no information is yet available on Ni and Zn redistribution and mobilization during seed germination. Due to the different physiological functions of these elements, and their associated transporter pathways, we expected differential tissue distribution and different modes of translocation of Ni and Zn during germination. This study used synchrotron X-ray fluorescence tomography techniques as well as planar elemental X-ray imaging to elucidate elemental (re)distribution at various stages of the germination process in contrasting accessions of N. caerulescens. The results show that Ni and Zn are both located primarily in the cotyledons of the emerging seedlings and Ni is highest in the ultramafic accessions (up to 0.15 wt%), whereas Zn is highest in the calamine accession (up to 600 μg g-1). The distribution of Ni and Zn in seeds was very similar, and neither element was translocated during germination. The Fe maps were especially useful to obtain spatial reference within the seeds, as it clearly marked the vasculature. This study shows how a multimodal combination of synchrotron techniques can be used to obtain powerful insights about the metal distribution in physically intact seeds and seedlings.

Original languageEnglish
Pages (from-to)1-9
JournalMetallomics : integrated biometal science
Volume14
Issue number5
DOIs
Publication statusPublished - 23 Jun 2022

Keywords

  • hyperaccumulator
  • reconstructions
  • seed
  • synchrotron
  • X-ray fluorescence microscopy
  • X-ray fluorescence tomography

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