Multicentre evaluation of a selective isolation protocol for detection of mcr-positive E. coli and Salmonella spp. in food-producing animals and meat

Agnès Perrin-Guyomard*, Sophie A. Granier, Jannice Schau Slettemeås, Muna Anjum, Luke Randall, Manal AbuOun, Natalie Pauly, Alexandra Irrgang, Jens Andre Hammerl, Jette Sejer Kjeldgaard, Anette Hammerum, Alessia Franco, Magdalena Skarżyńska, Ewelina Kamińska, Dariusz Wasyl, Cindy Dierikx, Stefan Börjesson, Yvon Geurts, Marisa Haenni, Kees Veldman

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

This study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive Escherichia coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving 12 laboratories from nine European countries. All participants applied the same methodology combining a multiplex PCR performed on DNA extracted from a pre-enrichment step, followed by a selective culture step on three commercially available chromogenic agar plates. The test panel was composed of two negative samples and four samples artificially contaminated with E. coli and Salmonella spp. respectively harbouring mcr-1 or mcr-3 and mcr-4 or mcr-5 genes. PCR screening resulted in a specificity of 100% and a sensitivity of 83%. Sensitivity of each agar medium to detect mcr-positive colistin-resistant E. coli or Salmonella spp. strains was 86% for CHROMID® Colistin R, 75% for CHROMagarTM COL-APSE and 70% for COLISTIGRAM. This combined method was effective to detect and isolate most of the E. coli or Salmonella spp. strains harbouring different mcr genes from food-producing animals and food products and might thus be used as a harmonized protocol for the screening of mcr genes in food-producing animals and food products in Europe.

Original languageEnglish
Pages (from-to)224-233
JournalLetters in Applied Microbiology
Volume75
Issue number2
Early online date2022
DOIs
Publication statusPublished - Aug 2022

Keywords

  • animals
  • Colistin resistance
  • E. coli
  • mcr
  • Salmonella
  • screening

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