Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

C. D'Agostino, N. Cook, I. Di Bartolo, F.M. Ruggeri, A. Berto, F. Martelli, M. Banks, P. Vasickova, P. Kralik, I. Pavlik, P. Kokkinos, A. Vantarakis, K. Söderberg, L. Maunula, K. Verhaelen, S. Rutjes, A.M. De Roda Husman, R.W. van der Hakze-van der Honing, W.H.M. van der Poel, A. KaupkeI. Kozyra, A. Rzezutka, J. Prodanov, S. Lazic, T. Petrovic, A. Carratala, R. Gironés, M. Diez-Valcarce, M. Hernandez, D. Rodriguez-Lazaro

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5×102 and 5×104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.
Original languageEnglish
Pages (from-to)1-7
JournalFood Analytical Methods
Volume5
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • pcr-based method
  • listeria-monocytogenes
  • international standard
  • enteric viruses
  • food
  • validation
  • water
  • contamination
  • environment
  • organisms

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