Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

C. D'Agostino; N. Cook; I. Di Bartolo; F.M. Ruggeri; A. Berto; F. Martelli; M. Banks; P. Vasickova; P. Kralik; I. Pavlik; P. Kokkinos; A. Vantarakis; K. Söderberg; L. Maunula; K. Verhaelen; S. Rutjes; A.M. De Roda Husman; R.W. van der Hakze-van der Honing; W.H.M. van der Poel; A. Kaupke; I. Kozyra; A. Rzezutka; J. Prodanov; S. Lazic; T. Petrovic; A. Carratala; R. Gironés; M. Diez-Valcarce; M. Hernandez; D. Rodriguez-Lazaro

doi:10.1007/s12161-011-9287-0

Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

C. D'Agostino, N. Cook, I. Di Bartolo, F.M. Ruggeri, A. Berto, F. Martelli, M. Banks, P. Vasickova, P. Kralik, I. Pavlik, P. Kokkinos, A. Vantarakis, K. Söderberg, L. Maunula, K. Verhaelen, S. Rutjes, A.M. De Roda Husman, R.W. van der Hakze-van der Honing, W.H.M. van der Poel, A. KaupkeI. Kozyra, A. Rzezutka, J. Prodanov, S. Lazic, T. Petrovic, A. Carratala, R. Gironés, M. Diez-Valcarce, M. Hernandez, D. Rodriguez-Lazaro

Virology & Molecular Biology

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5×102 and 5×104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.

Original language	English
Pages (from-to)	1-7
Journal	Food Analytical Methods
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/s12161-011-9287-0
Publication status	Published - 2012

Keywords

pcr-based method
listeria-monocytogenes
international standard
enteric viruses
food
validation
water
contamination
environment
organisms

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12161-011-9287-0

VITAL: Integrated Monitoring and Control of Foodborne Viruses in European Food Supply Chains
1/04/08 → 30/09/11
Project: EU research project
VITAL voedsel overdraagbare virussen (KB-06-001-014)
van der Poel, W. (Project Leader)
1/01/08 → 31/12/11
Project: LVVN project

Cite this

D'Agostino, C., Cook, N., Di Bartolo, I., Ruggeri, F. M., Berto, A., Martelli, F., Banks, M., Vasickova, P., Kralik, P., Pavlik, I., Kokkinos, P., Vantarakis, A., Söderberg, K., Maunula, L., Verhaelen, K., Rutjes, S., De Roda Husman, A. M., van der Hakze-van der Honing, R. W., van der Poel, W. H. M., ... Rodriguez-Lazaro, D. (2012). Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit. Food Analytical Methods, 5(1), 1-7. https://doi.org/10.1007/s12161-011-9287-0

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title = "Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit",

abstract = "The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5×102 and 5×104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.",

keywords = "pcr-based method, listeria-monocytogenes, international standard, enteric viruses, food, validation, water, contamination, environment, organisms",

author = "C. D'Agostino and N. Cook and {Di Bartolo}, I. and F.M. Ruggeri and A. Berto and F. Martelli and M. Banks and P. Vasickova and P. Kralik and I. Pavlik and P. Kokkinos and A. Vantarakis and K. S{\"o}derberg and L. Maunula and K. Verhaelen and S. Rutjes and {De Roda Husman}, A.M. and {van der Hakze-van der Honing}, R.W. and {van der Poel}, W.H.M. and A. Kaupke and I. Kozyra and A. Rzezutka and J. Prodanov and S. Lazic and T. Petrovic and A. Carratala and R. Giron{\'e}s and M. Diez-Valcarce and M. Hernandez and D. Rodriguez-Lazaro",

year = "2012",

doi = "10.1007/s12161-011-9287-0",

language = "English",

volume = "5",

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journal = "Food Analytical Methods",

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D'Agostino, C, Cook, N, Di Bartolo, I, Ruggeri, FM, Berto, A, Martelli, F, Banks, M, Vasickova, P, Kralik, P, Pavlik, I, Kokkinos, P, Vantarakis, A, Söderberg, K, Maunula, L, Verhaelen, K, Rutjes, S, De Roda Husman, AM, van der Hakze-van der Honing, RW , van der Poel, WHM, Kaupke, A, Kozyra, I, Rzezutka, A, Prodanov, J, Lazic, S, Petrovic, T, Carratala, A, Gironés, R, Diez-Valcarce, M, Hernandez, M & Rodriguez-Lazaro, D 2012, 'Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit', Food Analytical Methods, vol. 5, no. 1, pp. 1-7. https://doi.org/10.1007/s12161-011-9287-0

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T1 - Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

AU - D'Agostino, C.

AU - Cook, N.

AU - Di Bartolo, I.

AU - Ruggeri, F.M.

AU - Berto, A.

AU - Martelli, F.

AU - Banks, M.

AU - Vasickova, P.

AU - Kralik, P.

AU - Pavlik, I.

AU - Kokkinos, P.

AU - Vantarakis, A.

AU - Söderberg, K.

AU - Maunula, L.

AU - Verhaelen, K.

AU - Rutjes, S.

AU - De Roda Husman, A.M.

AU - van der Hakze-van der Honing, R.W.

AU - van der Poel, W.H.M.

AU - Kaupke, A.

AU - Kozyra, I.

AU - Rzezutka, A.

AU - Prodanov, J.

AU - Lazic, S.

AU - Petrovic, T.

AU - Carratala, A.

AU - Gironés, R.

AU - Diez-Valcarce, M.

AU - Hernandez, M.

AU - Rodriguez-Lazaro, D.

PY - 2012

Y1 - 2012

N2 - The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5×102 and 5×104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.

AB - The qualitative performance characteristics of a qPCR-based method to detect human adenoviruses in raspberries were determined through a collaborative trial involving 11 European laboratories. The method incorporated a sample process control (murine norovirus) and an internal amplification control. Trial sensitivity or correct identification of 25-g raspberry samples artificially contaminated with between 5×102 and 5×104 PFU was 98.5%; the accordance and concordance were 97.0%. The positive predictive value was 94.2%. The trial specificity or percentage correct identification of non-artificially contaminated samples was 69.7%; the accordance was 80.0% and the concordance was 61.7%. The negative predictive value was 100%. Application of a method for the detection of human adenoviruses in food samples could be useful for routine monitoring for food safety management. It would help to determine if a route of contamination exists from human source to food supply chain which pathogenic viruses such as norovirus and hepatitis A virus could follow.

KW - pcr-based method

KW - listeria-monocytogenes

KW - international standard

KW - enteric viruses

KW - food

KW - validation

KW - water

KW - contamination

KW - environment

KW - organisms

U2 - 10.1007/s12161-011-9287-0

DO - 10.1007/s12161-011-9287-0

M3 - Article

SN - 1936-9751

VL - 5

SP - 1

EP - 7

JO - Food Analytical Methods

JF - Food Analytical Methods

IS - 1

ER -

Multicenter Collaborative Trial Evaluation of a Method for Detection of Human Adenoviruses in Berry Fruit

Abstract

Keywords

UN SDGs

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Projects

VITAL: Integrated Monitoring and Control of Foodborne Viruses in European Food Supply Chains

VITAL voedsel overdraagbare virussen (KB-06-001-014)

Cite this