Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination., and XY body formation

F.A.T. de Vries, E. de Boer, M. Bosch, W.M. Baarends, M. Ooms, L. Yuan, J.G. Liu, A.A. van Zeeland, C. Heyting, A. Pastink

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257 Citations (Scopus)

Abstract

In meiotic prophase, synaptonemal complexes (SCs) closely appose homologous chromosomes (homologs) along their length. SCs are assembled from two axial elements (AEs), one along each homolog, which are connected by numerous transverse filaments (TFs). We disrupted the mouse gene encoding TF protein Sycp1 to analyze the role of TFs in meiotic chromosome behavior and recombination. Sycp1(-/-), mice are infertile, but otherwise healthy. Sycp1(-/-) spermatocytes form normal AEs, which align homologously, but do not synapse. Most Sycp1(-/-) spermatocytes arrest in pachynema, whereas a small proportion reaches diplonema, or, exceptionally, metaphase I. In leptotene Sycp1(-/-) spermatocytes, gamma H2AX (indicative of DNA damage, including double-strand breaks) appears normal. In pachynema, Sycp1(-/-) spermatocytes display a number of discrete gamma H2AX domains along each chromosome, whereas gamma H2AX disappears from autosomes in wild-type spermatocytes. RAD51/DMC1, RPA, and MSH4 foci (which mark early and intermediate steps in pairing/recombination) appear in similar numbers as in wild type, but do not all disappear, and MLH1 and MLH3 foci (which mark late steps in crossing over) are not formed. Crossovers were rare in metaphase I of Sycp1(-/-) mice. We propose that SYCP1 has a coordinating role, and ensures formation of crossovers. Unexpectedly, Sycp1(-/-) spermatocytes did not form XY bodies.
Original languageEnglish
Pages (from-to)1376-1389
JournalGenes and Development
Volume19
Issue number11
DOIs
Publication statusPublished - 2005

Keywords

  • double-strand breaks
  • mismatch repair proteins
  • chromosome synapsis
  • crossing-over
  • mammalian meiosis
  • axial elements
  • c-elegans
  • homolog
  • mice
  • localization

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    de Vries, F. A. T., de Boer, E., Bosch, M., Baarends, W. M., Ooms, M., Yuan, L., Liu, J. G., van Zeeland, A. A., Heyting, C., & Pastink, A. (2005). Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination., and XY body formation. Genes and Development, 19(11), 1376-1389. https://doi.org/10.1101/gad.329705