Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii

A.H.A.M. van Hoek, S. Mayrhofer, K.J. Domig, A.B. Flórez, M.S. Ammor, B. Mayo, H.J.M. Aarts

    Research output: Contribution to journalArticleAcademicpeer-review

    34 Citations (Scopus)

    Abstract

    For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third tet determinant was identified, coding for the efflux pump Tet(L).
    Original languageEnglish
    Pages (from-to)248-252
    JournalAntimicrobial Agents and Chemotherapy
    Volume52
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Keywords

    • megasphaera-elsdenii strains
    • swine production facilities
    • molecular characterization
    • antibiotic-resistance
    • ribosomal protection
    • sequence
    • tet(w)
    • susceptibility
    • residues
    • pyogenes

    Fingerprint Dive into the research topics of 'Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii'. Together they form a unique fingerprint.

    Cite this