Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii

A.H.A.M. van Hoek, S. Mayrhofer, K.J. Domig, A.B. Flórez, M.S. Ammor, B. Mayo, H.J.M. Aarts

    Research output: Contribution to journalArticleAcademicpeer-review

    33 Citations (Scopus)

    Abstract

    For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third tet determinant was identified, coding for the efflux pump Tet(L).
    Original languageEnglish
    Pages (from-to)248-252
    JournalAntimicrobial Agents and Chemotherapy
    Volume52
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Bifidobacterium
    Tetracycline
    Genes
    Tetracycline Resistance
    Gene Order
    Genetic Recombination
    Open Reading Frames
    Sequence Analysis
    Lactobacillus johnsonii

    Keywords

    • megasphaera-elsdenii strains
    • swine production facilities
    • molecular characterization
    • antibiotic-resistance
    • ribosomal protection
    • sequence
    • tet(w)
    • susceptibility
    • residues
    • pyogenes

    Cite this

    van Hoek, A. H. A. M., Mayrhofer, S., Domig, K. J., Flórez, A. B., Ammor, M. S., Mayo, B., & Aarts, H. J. M. (2008). Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii. Antimicrobial Agents and Chemotherapy, 52(1), 248-252. https://doi.org/10.1128/AAC.00714-07
    van Hoek, A.H.A.M. ; Mayrhofer, S. ; Domig, K.J. ; Flórez, A.B. ; Ammor, M.S. ; Mayo, B. ; Aarts, H.J.M. / Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii. In: Antimicrobial Agents and Chemotherapy. 2008 ; Vol. 52, No. 1. pp. 248-252.
    @article{ea225bbc6dec4b648d748c72aa919da8,
    title = "Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii",
    abstract = "For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third tet determinant was identified, coding for the efflux pump Tet(L).",
    keywords = "megasphaera-elsdenii strains, swine production facilities, molecular characterization, antibiotic-resistance, ribosomal protection, sequence, tet(w), susceptibility, residues, pyogenes",
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    year = "2008",
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    language = "English",
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    van Hoek, AHAM, Mayrhofer, S, Domig, KJ, Flórez, AB, Ammor, MS, Mayo, B & Aarts, HJM 2008, 'Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii' Antimicrobial Agents and Chemotherapy, vol. 52, no. 1, pp. 248-252. https://doi.org/10.1128/AAC.00714-07

    Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii. / van Hoek, A.H.A.M.; Mayrhofer, S.; Domig, K.J.; Flórez, A.B.; Ammor, M.S.; Mayo, B.; Aarts, H.J.M.

    In: Antimicrobial Agents and Chemotherapy, Vol. 52, No. 1, 2008, p. 248-252.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Mosaic tetracycline Genes and their Flanking Regions in Bifidobacterium thermophilum and lactobacillus johnsonii

    AU - van Hoek, A.H.A.M.

    AU - Mayrhofer, S.

    AU - Domig, K.J.

    AU - Flórez, A.B.

    AU - Ammor, M.S.

    AU - Mayo, B.

    AU - Aarts, H.J.M.

    PY - 2008

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    N2 - For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third tet determinant was identified, coding for the efflux pump Tet(L).

    AB - For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third tet determinant was identified, coding for the efflux pump Tet(L).

    KW - megasphaera-elsdenii strains

    KW - swine production facilities

    KW - molecular characterization

    KW - antibiotic-resistance

    KW - ribosomal protection

    KW - sequence

    KW - tet(w)

    KW - susceptibility

    KW - residues

    KW - pyogenes

    U2 - 10.1128/AAC.00714-07

    DO - 10.1128/AAC.00714-07

    M3 - Article

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    EP - 252

    JO - Antimicrobial Agents and Chemotherapy

    JF - Antimicrobial Agents and Chemotherapy

    SN - 0066-4804

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    ER -