Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division

Renze Heidstra*, David Welch, Ben Scheres

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

194 Citations (Scopus)

Abstract

In the Arabidopsis root meristem, ground tissue stem cell daughters perform an asymmetric division to form endodermis and cortex. The putative transcription factors SCARECROW (SCR) and SHORTROOT (SHR) regulate this radial patterning event, and the mixed cell fate in scr mutants suggests an atypical role of the SCR gene in asymmetric cell division. Here we use a newly developed site-specific gene activation/deletion system in which induced clones are positively marked with green fluorescent protein (GFP). Using this system, we show that SCR acts cell-autonomously to control asymmetric cell division within the ground tissue. We provide evidence that SCR gene expression is under autoregulatory control, that SCR limits SHR movement, and that transient SCR action is sufficient to separate endodermis and cortex fates by asymmetric cell division.

Original languageEnglish
Pages (from-to)1964-1969
Number of pages6
JournalGenes and Development
Volume18
Issue number16
DOIs
Publication statusPublished - 15 Aug 2004
Externally publishedYes

Keywords

  • Asymmetric cell division
  • CRE/lox
  • Meristem
  • Pattern formation

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