Abstract
Twenty four Meloidogyne populations from Andean fruits (Cyphomandra betacea, Solanum quitoense, Passiflore ligularis) and Musa plants
occurring in Colombia were identified using different approaches:
morphological and morphometrical analyses by light (LM) and scanning
electron microscopy (SEM), species-specific primers, protein analyses, molecular analyses of sequences and phylogenetic analyses.
Morphometric and/or morphological features allowed separation of populations into species groups as a first step towards
identification. Although purification of populations is recommended
as a second step in the identification, only three species could be
successfully purified. However, to overcome the mixed species problem
for all other species, Meloidogyne identification was based on a
single young egg-laying female (protein analysis) and DNA (for
species-specific PCR and sequence analyses) was always extracted from
a single juvenile. Six known species were identified: M. incognita, M.
javanica, M. arenaria, M. hapla, M. konaensis and M. paranaensis. The
last two species had never been recorded previously in Colombia but
since they show morphological features of M. incognita and M. arenaria
they were probably misidentified in the past. Isozyme analysis also
recovered two phenotypes that could not be connected to known
Meloidogyne species. The first phenotype represents a new species
that is currently under investigations of researchers from Holland and
Brazil and, additional specimens of the second phenotype are needed
for accurate morphological and morphometrical studies.
Original language | English |
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Publication status | Published - 2010 |
Event | Onta Meeting, Quito, Ecuador - Duration: 2 Oct 2010 → 9 Oct 2010 |
Conference
Conference | Onta Meeting, Quito, Ecuador |
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Period | 2/10/10 → 9/10/10 |