Moorella stamsii sp. nov., a new anaerobic thermophilic hydrogenogenic carboxydotroph isolated from digester sludge

J.I. Alves, A.H. van Gelder, M.M. Alves, D.Z. Sousa, C.M. Plugge

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

A novel anaerobic, thermophilic, carbon monoxide-utilizing bacterium, strain E3-O, was isolated from anaerobic sludge of a municipal solid waste digester. Cells were straight rods, 0.6 to 1µm in diameter and 2 to 3 µm in length, growing as single cells or in pairs. Cells formed round terminal endospores. The temperature range for growth was 50 to 70°C, with an optimum at 65°C. The pH range for growth was 5.7 to 8.0, with an optimum at 7.5. Strain E3-O had the capability to ferment various sugars, such as fructose, galactose, glucose, mannose, raffinose, ribose, sucrose and xylose, producing mainly H2 and acetate. In addition, the isolate was able to grow with CO as the sole carbon and energy source. CO oxidation was coupled to H2 and CO2 formation. The G+C content of the genomic DNA was 54.6 mol %. Based on 16S rRNA gene sequence analysis, this bacterium is most closely related to Moorella glycerini (97% sequence identity). Based on the physiological features and phylogenetic analysis, it is proposed that strain E3-O should be classified in the genus Moorella as a new species, Moorella stamsii. The type strain of Moorella stamsii is E3-OT (=DMS 26271T = CGMCC 1.5181T)
LanguageEnglish
Pages4072-4076
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue number11
DOIs
Publication statusPublished - 2013

Fingerprint

Moorella
Sewage
sludge
Carbon Monoxide
bacterium
dimethylsulfide
new species
sucrose
Moorella glycerini
carbon monoxide
municipal solid waste
sugar
acetate
genomics
glucose
Raffinose
Bacteria
phylogenetics
Solid Waste
DNA

Keywords

  • gradient gel-electrophoresis
  • carbon-monoxide
  • growth
  • metabolism
  • bacteria

Cite this

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title = "Moorella stamsii sp. nov., a new anaerobic thermophilic hydrogenogenic carboxydotroph isolated from digester sludge",
abstract = "A novel anaerobic, thermophilic, carbon monoxide-utilizing bacterium, strain E3-O, was isolated from anaerobic sludge of a municipal solid waste digester. Cells were straight rods, 0.6 to 1µm in diameter and 2 to 3 µm in length, growing as single cells or in pairs. Cells formed round terminal endospores. The temperature range for growth was 50 to 70°C, with an optimum at 65°C. The pH range for growth was 5.7 to 8.0, with an optimum at 7.5. Strain E3-O had the capability to ferment various sugars, such as fructose, galactose, glucose, mannose, raffinose, ribose, sucrose and xylose, producing mainly H2 and acetate. In addition, the isolate was able to grow with CO as the sole carbon and energy source. CO oxidation was coupled to H2 and CO2 formation. The G+C content of the genomic DNA was 54.6 mol {\%}. Based on 16S rRNA gene sequence analysis, this bacterium is most closely related to Moorella glycerini (97{\%} sequence identity). Based on the physiological features and phylogenetic analysis, it is proposed that strain E3-O should be classified in the genus Moorella as a new species, Moorella stamsii. The type strain of Moorella stamsii is E3-OT (=DMS 26271T = CGMCC 1.5181T)",
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author = "J.I. Alves and {van Gelder}, A.H. and M.M. Alves and D.Z. Sousa and C.M. Plugge",
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Moorella stamsii sp. nov., a new anaerobic thermophilic hydrogenogenic carboxydotroph isolated from digester sludge. / Alves, J.I.; van Gelder, A.H.; Alves, M.M.; Sousa, D.Z.; Plugge, C.M.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. 11, 2013, p. 4072-4076.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Moorella stamsii sp. nov., a new anaerobic thermophilic hydrogenogenic carboxydotroph isolated from digester sludge

AU - Alves, J.I.

AU - van Gelder, A.H.

AU - Alves, M.M.

AU - Sousa, D.Z.

AU - Plugge, C.M.

PY - 2013

Y1 - 2013

N2 - A novel anaerobic, thermophilic, carbon monoxide-utilizing bacterium, strain E3-O, was isolated from anaerobic sludge of a municipal solid waste digester. Cells were straight rods, 0.6 to 1µm in diameter and 2 to 3 µm in length, growing as single cells or in pairs. Cells formed round terminal endospores. The temperature range for growth was 50 to 70°C, with an optimum at 65°C. The pH range for growth was 5.7 to 8.0, with an optimum at 7.5. Strain E3-O had the capability to ferment various sugars, such as fructose, galactose, glucose, mannose, raffinose, ribose, sucrose and xylose, producing mainly H2 and acetate. In addition, the isolate was able to grow with CO as the sole carbon and energy source. CO oxidation was coupled to H2 and CO2 formation. The G+C content of the genomic DNA was 54.6 mol %. Based on 16S rRNA gene sequence analysis, this bacterium is most closely related to Moorella glycerini (97% sequence identity). Based on the physiological features and phylogenetic analysis, it is proposed that strain E3-O should be classified in the genus Moorella as a new species, Moorella stamsii. The type strain of Moorella stamsii is E3-OT (=DMS 26271T = CGMCC 1.5181T)

AB - A novel anaerobic, thermophilic, carbon monoxide-utilizing bacterium, strain E3-O, was isolated from anaerobic sludge of a municipal solid waste digester. Cells were straight rods, 0.6 to 1µm in diameter and 2 to 3 µm in length, growing as single cells or in pairs. Cells formed round terminal endospores. The temperature range for growth was 50 to 70°C, with an optimum at 65°C. The pH range for growth was 5.7 to 8.0, with an optimum at 7.5. Strain E3-O had the capability to ferment various sugars, such as fructose, galactose, glucose, mannose, raffinose, ribose, sucrose and xylose, producing mainly H2 and acetate. In addition, the isolate was able to grow with CO as the sole carbon and energy source. CO oxidation was coupled to H2 and CO2 formation. The G+C content of the genomic DNA was 54.6 mol %. Based on 16S rRNA gene sequence analysis, this bacterium is most closely related to Moorella glycerini (97% sequence identity). Based on the physiological features and phylogenetic analysis, it is proposed that strain E3-O should be classified in the genus Moorella as a new species, Moorella stamsii. The type strain of Moorella stamsii is E3-OT (=DMS 26271T = CGMCC 1.5181T)

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KW - growth

KW - metabolism

KW - bacteria

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SP - 4072

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SN - 1466-5026

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ER -