Moorella caeni sp. nov., isolated from thermophilic anaerobic sludge from a methanol-fed reactor

Nicolás Vecchini Santaella; Diana Z. Sousa; Alfons J.M. Stams

doi:10.1099/ijsem.0.005905

Moorella caeni sp. nov., isolated from thermophilic anaerobic sludge from a methanol-fed reactor

Nicolás Vecchini Santaella, Diana Z. Sousa^*, Alfons J.M. Stams

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Strain AMP^T has been previously suggested as a strain of the species Moorella thermoacetica Jiang et al. 2009 (based on the high 16S rRNA gene identity, 98.3 %). However, genome-based phylogenetic analysis of strain AMP^T reveals that this bacterium is in fact a novel species of the genus Moorella. Genome relatedness indices between strain AMP^T and Moorella thermoacetica DSM 521^T were below the minimum threshold values required to consider them members of the same species (digital DNA–DNA hybridization, 52.2 % (<70%); average nucleotide identity, 93.2 % (<95%)). Based on phylogenetic and phenotypic results we rec-ommend that strain AMP^T (DSM 21394^T=JCM 35360^T) should be classified as representing new species, for which we propose the name Moorella caeni sp. nov.

Original language	English
Article number	005905
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	73
Issue number	5
DOIs	https://doi.org/10.1099/ijsem.0.005905
Publication status	Published - 1 May 2023

Keywords

acetogen
carbon monoxide
methanol
Moorella
Moorella caeni sp. nov.

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https://edepot.wur.nl/635583Licence: CC BY

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title = "Moorella caeni sp. nov., isolated from thermophilic anaerobic sludge from a methanol-fed reactor",

abstract = "Strain AMPT has been previously suggested as a strain of the species Moorella thermoacetica Jiang et al. 2009 (based on the high 16S rRNA gene identity, 98.3 %). However, genome-based phylogenetic analysis of strain AMPT reveals that this bacterium is in fact a novel species of the genus Moorella. Genome relatedness indices between strain AMPT and Moorella thermoacetica DSM 521T were below the minimum threshold values required to consider them members of the same species (digital DNA–DNA hybridization, 52.2 % (<70%); average nucleotide identity, 93.2 % (<95%)). Based on phylogenetic and phenotypic results we rec-ommend that strain AMPT (DSM 21394T=JCM 35360T) should be classified as representing new species, for which we propose the name Moorella caeni sp. nov.",

keywords = "acetogen, carbon monoxide, methanol, Moorella, Moorella caeni sp. nov.",

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doi = "10.1099/ijsem.0.005905",

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T1 - Moorella caeni sp. nov., isolated from thermophilic anaerobic sludge from a methanol-fed reactor

AU - Santaella, Nicolás Vecchini

AU - Sousa, Diana Z.

AU - Stams, Alfons J.M.

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N2 - Strain AMPT has been previously suggested as a strain of the species Moorella thermoacetica Jiang et al. 2009 (based on the high 16S rRNA gene identity, 98.3 %). However, genome-based phylogenetic analysis of strain AMPT reveals that this bacterium is in fact a novel species of the genus Moorella. Genome relatedness indices between strain AMPT and Moorella thermoacetica DSM 521T were below the minimum threshold values required to consider them members of the same species (digital DNA–DNA hybridization, 52.2 % (<70%); average nucleotide identity, 93.2 % (<95%)). Based on phylogenetic and phenotypic results we rec-ommend that strain AMPT (DSM 21394T=JCM 35360T) should be classified as representing new species, for which we propose the name Moorella caeni sp. nov.

AB - Strain AMPT has been previously suggested as a strain of the species Moorella thermoacetica Jiang et al. 2009 (based on the high 16S rRNA gene identity, 98.3 %). However, genome-based phylogenetic analysis of strain AMPT reveals that this bacterium is in fact a novel species of the genus Moorella. Genome relatedness indices between strain AMPT and Moorella thermoacetica DSM 521T were below the minimum threshold values required to consider them members of the same species (digital DNA–DNA hybridization, 52.2 % (<70%); average nucleotide identity, 93.2 % (<95%)). Based on phylogenetic and phenotypic results we rec-ommend that strain AMPT (DSM 21394T=JCM 35360T) should be classified as representing new species, for which we propose the name Moorella caeni sp. nov.

KW - acetogen

KW - carbon monoxide

KW - methanol

KW - Moorella

KW - Moorella caeni sp. nov.

U2 - 10.1099/ijsem.0.005905

DO - 10.1099/ijsem.0.005905

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Moorella caeni sp. nov., isolated from thermophilic anaerobic sludge from a methanol-fed reactor

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Keywords

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