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Abstract
Subcellular monoterpene biosynthesis capacity based on local geranyl diphosphate (GDP)
availability or locally boosted GDP production was determined for plastids, cytosol and mitochondria.
A geraniol synthase (GES) was targeted to plastids, cytosol, or mitochondria. Transient
expression in Nicotiana benthamiana indicated local GDP availability for each compartment
but resulted in different product levels. A GDP synthase from Picea abies (PaGDPS1) was
shown to boost GDP production. PaGDPS1 was also targeted to plastids, cytosol or mitochondria
and PaGDPS1 and GES were coexpressed in all possible combinations.
Geraniol and geraniol-derived products were analyzed by GC-MS and LC-MS, respectively.
GES product levels were highest for plastid-targeted GES, followed by mitochondrial- and
then cytosolic-targeted GES. For each compartment local boosting of GDP biosynthesis
increased GES product levels.
GDP exchange between compartments is not equal: while no GDP is exchanged from the
cytosol to the plastids, 100% of GDP in mitochondria can be exchanged to plastids, while only
7% of GDP from plastids is available for mitochondria. This suggests a direct exchange mechanism
for GDP between plastids and mitochondria. Cytosolic PaGDPS1 competes with plastidial
GES activity, suggesting an effective drain of isopentenyl diphosphate from the plastids
to the cytosol.
Original language | English |
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Pages (from-to) | 679-690 |
Journal | New Phytologist |
Volume | 209 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- Nicotiana benthamiana
- Cytosol
- Geranyl diphosphate synthase (GDPS)
- Mitochondria
- Plastids
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Dive into the research topics of 'Monoterpene biosynthesis potential of plant subcellular compartments'. Together they form a unique fingerprint.Projects
- 1 Finished
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SMARTCELL: Rational design of plant systems for sustainable generation of value-added industrial products
1/01/09 → 30/09/13
Project: EU research project