Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3

R.M.E. Vos; I.J. Lutkeschipholt; P.M. Boonekamp; A. Schots; H. Pomp; P.J. van Bladeren; J.H. Koeman

doi:10.1089/hyb.1989.8.475

Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3

R.M.E. Vos, I.J. Lutkeschipholt, P.M. Boonekamp, A. Schots, H. Pomp, P.J. van Bladeren, J.H. Koeman

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rac isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3.
Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.

Original language	English
Pages (from-to)	475-480
Journal	Hybridoma
Volume	8
DOIs	https://doi.org/10.1089/hyb.1989.8.475
Publication status	Published - 1989

Access to Document

10.1089/hyb.1989.8.475

https://edepot.wur.nl/434371Licence: Taverne

Cite this

@article{8f3890c52c5b4235b48c3069743c57d4,

title = "Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3",

abstract = "Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rac isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3.Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.",

author = "R.M.E. Vos and I.J. Lutkeschipholt and P.M. Boonekamp and A. Schots and H. Pomp and {van Bladeren}, P.J. and J.H. Koeman",

year = "1989",

doi = "10.1089/hyb.1989.8.475",

language = "English",

volume = "8",

pages = "475--480",

journal = "Hybridoma",

issn = "1554-0014",

publisher = "Mary Ann Liebert",

}

TY - JOUR

T1 - Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3

AU - Vos, R.M.E.

AU - Lutkeschipholt, I.J.

AU - Boonekamp, P.M.

AU - Schots, A.

AU - Pomp, H.

AU - van Bladeren, P.J.

AU - Koeman, J.H.

PY - 1989

Y1 - 1989

N2 - Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rac isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3.Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.

AB - Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rac isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3.Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.

U2 - 10.1089/hyb.1989.8.475

DO - 10.1089/hyb.1989.8.475

M3 - Article

SN - 1554-0014

VL - 8

SP - 475

EP - 480

JO - Hybridoma

JF - Hybridoma

ER -

Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3

Abstract

Access to Document

Fingerprint

Cite this